D1667
Monoclonal Anti-Dynein (Heavy Chain) antibody produced in mouse
clone 440.4, ascites fluid
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About This Item
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biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
440.4, monoclonal
mol wt
antigen 440 kDa
contains
15 mM sodium azide
species reactivity
human, chicken
technique(s)
indirect ELISA: suitable
western blot: 1:100 using a chick brain extract or Kinesin enriched rat brain extract
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... DNAH1(25981) , DNAH2(146754) , DNAH3(55567) , DNAH5(1767)
General description
Monoclonal Anti-Dynein (Heavy Chain) (mouse IgG2a isotype) is derived from the 440.4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Dynein axonemal heavy chain 1 (DNAH1) encodes for an inner dynein heavy chain. This gene is mapped to the human chromosome location 3p21.1. DNAH1 is expressed in the testis and other ciliated cells. Dyneins are composed of two or three distinct heavy chains, three intermediate chains and at least four light chains.
Immunogen
cytoplasmic dynein from chicken embryo brain.
Application
Monoclonal Anti-Dynein (Heavy Chain) antibody produced in mouse has been used in:
- indirect immunofluorescence at 1:50 dilution
- immunocytochemistry
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- dot blot
Biochem/physiol Actions
Dynein has also been implicated in cytoplasmic motile functions, including chromosomal movement, retrograde organelle and axonal transport. It also regulates the endocytic pathway and the organization of the Golgi apparatus. Mutations in dynein axonemal heavy chain 1 (DNAH1) are associated with primary ciliary dyskinesia and multiple morphological anomalies of the flagella (MMAF) that leads to asthenozoospermia and male infertility.
Dynein is a motor protein that regulates the movement of organelles to the ‘minus′ end of microtubules. These motor proteins are also involved in spindle organization, retrograde transport and chromosome movement
Physical form
Suppied as ascites fluid with 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Mariem Ben Khelifa et al.
American journal of human genetics, 94(1), 95-104 (2013-12-24)
Ten to fifteen percent of couples are confronted with infertility and a male factor is involved in approximately half the cases. A genetic etiology is likely in most cases yet only few genes have been formally correlated with male infertility.
Rollin W Robinson et al.
Histochemistry and cell biology, 122(1), 1-5 (2004-06-09)
Using a new fixation solution, CytoSkelFix, it is now possible to obtain superior fixation and thus resolution of cytoskeletal components using immunofluorescence and fluorescence microscopy. This fixative combines rapid cell penetration and cellular crosslinking of proteins such that both preservation
E R Steuer et al.
Nature, 345(6272), 266-268 (1990-05-17)
What is the origin of the forces generating chromosome and spindle movements in mitosis? Both microtubule dynamics and microtubule-dependent motors have been proposed as the source of these motor forces. Cytoplasmic dynein and kinesin are two soluble proteins that power
Stephanie K Carnes et al.
Journal of virology, 92(20) (2018-08-03)
Human immunodeficiency virus type 1 (HIV-1) infection depends on efficient intracytoplasmic transport of the incoming viral core to the target cell nucleus. Evidence suggests that this movement is facilitated by the microtubule motor dynein, a large multiprotein complex that interacts
X Wang et al.
Clinical genetics, 91(2), 313-321 (2016-08-31)
This study aimed to investigate the genetic pathogeny of multiple morphological anomalies of the flagella (MMAF), which is a genetically heterogeneous disorder leading to male infertility. Nine patients with severe asthenozoospermia caused by MMAF were recruited. Whole genome sequencing and
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