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product name
SCP, 89101302
biological source
ovine brain
growth mode
Adherent
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Ovine brain choroid plexus
Cell Line Description
Initiated from the choroid plexus of an ovine brain. The cells support propagation of Visna virus, strain 1514 with resulting cytopathic effects. Tests have indicated that the cell line produces both detectable BVDV antigens and infectious BVDV.
Application
Virus studies: Visna virus
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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