ONE58 cell line human
10092313, human esophagus, Epithelial-like: cells are spindle-shaped with few vacuoles.
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About This Item
UNSPSC Code:
41106514
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product name
ONE58 cell line human, 10092313
biological source
human esophagus
description
Human malignant mesothelioma
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial-like: cells are spindle-shaped with few vacuoles.
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human malignant mesothelioma
Cell Line Description
This cell line was derived from the pleural fluid of a patient with malignant mesothelioma. The patient had known exposure to crocidolite asbestos. ONE58 is tumourigenic in nude mice. Cells express cytokeratin and epithelial membrane antigen (EMA) but not mucin. Cells are spindle shaped with few vacuoles. Cell Bank Australia reference number: CBA0143
Application
Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.
Culture Medium
RPMI 1640 (with 2mM L-Glutamine+25mM HEPES) + 5% FCS
Subculture Routine
Optimal split ratio 1:4 (seeding density 1.2 x 104 cells/cm2).Cells incubated at 37°C with 5% CO2.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.
Other Notes
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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Richard Lake - University of Western Australia, Australia
Certificates of Analysis (COA)
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