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Key Documents

C6144

Sigma-Aldrich

Chlorophyll a

Powder

Synonym(s):

Pheophytin; universal photosynthetic pigment

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About This Item

Empirical Formula (Hill Notation):
C55H72MgN4O5
CAS Number:
Molecular Weight:
893.49
Beilstein:
3586237
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

Chlorophyll a, from Anacystis nidulans algae

biological source

Anacystis nidulans algae

Quality Level

form

powder

impurities

chlorophyll b, free

color

blue to very dark blue

mp

150-153 °C

solubility

diethyl ether: 0.1 mg/10 mL

ε (extinction coefficient)

11.1-12.1 × 104 at 428-430 nm in diethyl ether
8.3-9.4 × 104 at 660-662 nm in diethyl ether

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

CCc1c(C)c2cc3c(C=C)c(C)c4cc5nc([C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@@H]5C)c6[C@@H](C(=O)OC)C(=O)c7c(C)c(cc1n2)n([Mg]n34)c67

InChI

1S/C55H73N4O5.Mg/c1-13-39-35(8)42-28-44-37(10)41(24-25-48(60)64-27-26-34(7)23-17-22-33(6)21-16-20-32(5)19-15-18-31(3)4)52(58-44)50-51(55(62)63-12)54(61)49-38(11)45(59-53(49)50)30-47-40(14-2)36(9)43(57-47)29-46(39)56-42;/h13,26,28-33,37,41,51H,1,14-25,27H2,2-12H3,(H-,56,57,58,59,61);/q-1;+2/p-1/b34-26+;/t32-,33-,37+,41+,51-;/m1./s1

InChI key

ATNHDLDRLWWWCB-AENOIHSZSA-M

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Application

A procedure has been described for harvesting native light-harvesting complex IIb (LHCIIb).
Chlorophyll a has been used as a standard for the calibration of fluorometer.

Biochem/physiol Actions

Chlorophyll a is an essential pigment in oxygenic photosynthesis. In photosystems, it is present as an antenna pigment as well as in reaction center I and II. Chlorophyll a is a blue-green pigment which absorbs light in 400-450nm and 640-660nm range. In photosynthesis, the energy absorbed by chlorophyll transforms carbon dioxide and water into carbohydrates and oxygen. When chlorophyll absorbs light energy, an electron in chlorophyll is excited from a lower energy state to a higher energy state. In this higher energy state, the electron is more readily transferred to another molecule. This starts a chain of electron-transfer steps, which ends with an electron transferred to carbon dioxide.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.
Sealed ampule.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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