C0618
Anti-Calsequestrin-1 antibody produced in rabbit
~1.6 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-Aspartactin, Anti-CALMITINE, Anti-CASQ1, Anti-Calsequestrin-skeletal muscle isoform
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About This Item
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biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~60 kDa
species reactivity
canine, mouse, human, rat
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
concentration
~1.6 mg/mL
technique(s)
western blot: 0.5-1 μg/mL using extracts of rat skeletal muscle S1 fraction
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CASQ1(844)
mouse ... Casq1(12372)
General description
Calsequestrin is expected to modulate ryanodine receptor (RyR) signaling and Ca2+ release with the help of triadin and junction proteins.
Immunogen
synthetic peptide corresponding to amino acids 66-84 located near the N-terminus of mouse calsequestrin-1, conjugated to KLH. This sequence is identical in human, rat, and dog calsequestrin-1 and highly conserved in bovine (single amino acid substitution). This sequence is not found in calsequestrin-2.
Application
Anti-Calsequestrin-1 antibody produced in rabbit has been used in immunofluorescence.
Anti-Calsequestrin-1 antibody produced in rabbit is suitable for western blotting at a concentration of 0.5-1μg/mL using extracts of rat skeletal muscle S1 fraction.
Biochem/physiol Actions
Calsequestrin-1 is a putative auto-antigen associated with eye muscle inflammation in Graves′ disease. Calsequestrin-2 (55 kDa) is found in the SR′s terminal cisternae luminal space of cardiac, slow skeletal muscle cells and may be in platelets. The major Ca2+ binding protein in cardiac and skeletal muscle is a high-capacity, low-affinity Ca2+ binding glycoprotein, which functions as an internal Ca2+ store in the lumen of the SR. It helps in controlling the smooth-muscle contraction. Calsequestrin is expected to modulate ryanodine receptor (RyR) signaling and Ca2+ release with the help of triadin and junction proteins.†
Target description
Calsequestrin (CS also known as CSQ), the major Ca2+ binding protein in cardiac and skeletal muscle, is a high-capacity, low-affinity Ca2+ binding glycoprotein, which function as an internal Ca2+ store in the lumen of the SR.1-3 In mammals, two forms of the protein exist, calsequestrin-1 (CASQ-1) and calsequestrin-2 (CASQ-2), which encode the fast-twitch skeletal muscle and cardiac calsequestrin, respectively. Calsequestrin-1 is found in the SRs terminal cisternae luminal space of fast skeletal muscle cells. Calsequestrin-1 is a putative autoantigen associated with eye muscle inflammation in Graves′ disease.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Calsequestrin, triadin and more: the molecules that modulate calcium release in cardiac and skeletal muscle
Rios E and Gyorke S
The Journal of Physiology, 587(Pt 13), 3069-3069 (2009)
K Gunji et al.
Autoimmunity, 29(1), 1-9 (1999-03-03)
It is generally accepted that thyroid-associated ophthalmopathy (TAO) is an autoimmune disease of the eye muscle (EM) and the surrounding orbital connective tissue in which circulating antibodies play an important role. Antibodies against EM membrane proteins of 63-67kDa mol. wt.
Identification of cellular pathogenicity markers for SIL1 mutations linked to Marinesco-Sjogren syndrome
Gatz C, et al.
Frontiers in Neurology, 10 (2019)
B Gopinath et al.
Clinical and experimental immunology, 145(1), 56-62 (2006-06-24)
We have identified several eye muscle antigens and studied the significance of the corresponding serum autoantibodies in patients with Graves' disease. Of these antigens, only calsequestrin is expressed more in eye muscle than other skeletal muscles, which could explain at
Natalia Kraeva et al.
Anesthesiology, 118(2), 344-349 (2013-03-06)
Malignant hyperthermia (MH, MIM# 145600) is a complex pharmacogenetic disorder that is manifested in predisposed individuals as a potentially lethal reaction to volatile anesthetics and depolarizing muscle relaxants. Studies of CASQ1-null mice have shown that CASQ1, encoding calsequestrin 1, the
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