Skip to Content
Merck
All Photos(2)

Documents

A8919

Sigma-Aldrich

Anti-Goat IgG (whole molecule)–Peroxidase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti Goat Hrp Sigma

Sign Into View Organizational & Contract Pricing


About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

conjugate

peroxidase conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:30,000
dot blot: 1:2,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
western blot (chemiluminescent): 1:160,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Looking for similar products? Visit Product Comparison Guide

General description

Immunoglobulin is a glycoprotein, with two heavy chain and two light chain connected by disulfide bond. Immunoglobulin g (IgG) is a major class of immunoglobulin. Goat IgG has two subclasses- IgG1 and IgG2. Rabbit anti-Goat IgG is then conjugated to peroxidase by protein cross linking with 0.2% glutaraldehyde. The conjugate is provided as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT as a preservative.

Immunogen

Purified goat IgG

Application

Anti-Goat IgG (whole molecule)-Peroxidase antibody produced in rabbit has been used in immunoblotting and immunohistochemistry.

Biochem/physiol Actions

Immunoglobulin mainly helps in immune defense. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reaction.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Distinct roles of the phosphatidate phosphatases lipin 1 and 2 during adipogenesis and lipid droplet biogenesis in 3T3-L1 cells
Sembongi H, et al.
The Journal of Biological Chemistry, 288(48), 34502-34513 (2013)
OTX2 is a therapeutic target for retinoblastoma and may function as a common factor between C-MYC, CRX, and phosphorylated RB pathways
Li J, et al.
International Journal of Oncology, 47(5), 1703-1710 (2015)
Zein Shaban Ibrahim
Experimental and therapeutic medicine, 10(5), 1976-1982 (2015-12-08)
Bile acids are considered to promote carcinogenesis. Cytochrome P450 1A1 (CYP1A1) plays a critical role in the biotransformation of drugs and procarcinogens. This study aimed to investigate the ability of bile acids to modulate CYP1A1 expression. Treatment of HepG2 cells
Deanna L Plubell et al.
Molecular & cellular proteomics : MCP, 16(5), 873-890 (2017-03-23)
The lack of high-throughput methods to analyze the adipose tissue protein composition limits our understanding of the protein networks responsible for age and diet related metabolic response. We have developed an approach using multiple-dimension liquid chromatography tandem mass spectrometry and
Ascorbic acid spares alpha-tocopherol and decreases lipid peroxidation in neuronal cells
Li X, et al.
Biochemical and biophysical research communications, 305(3), 656-661 (2003)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service