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B8756

Millipore

Bile salts

suitable for microbiology

Synonym(s):

Bile acids sodium salt, cholic acid-deoxycholic acid sodium salt mixture

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About This Item

UNSPSC Code:
41106212
NACRES:
NA.85

sterility

non-sterile

Quality Level

form

powder

solubility

distilled water: freely soluble

application(s)

microbiology

storage temp.

10-30°C

General description

Bile salt is an organic sodium salt with a conjugate of any of the bile acids along with either glycine or taurine. Biles salts are composed of four different kinds of bile acids - cholic, deoxycholic, chenodeoxycholic and lithocholic acids.Bile salts are extracted under controlled conditions from purified fresh bile, to be used in bacteriological culture media, where it behaves as a selective inhibitory agent that enables isolation and identification of pathogens. For example, in MacConkey Agar/Broth.

Application

Bile salts may be used as one of the selective agents to prevent the growth of injured Escherichia coli cells, in an experiment done in order to identify the specific agent in the selective media which would inhibit the growth of the injured Escherichia coli ML30 cells. It may also be used for identification of isolates of Vibrio (Beneckea) vulnificus which is a halophilic bacterium, found responsible for causing several human infections.

Other Notes

Mixture of sodium cholate and sodium deoxycholate

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Molecular microbiology, 85(1), 107-121 (2012-05-11)
Shigella flexneri is a Gram-negative pathogen that invades the colonic epithelium. While invasion has been thoroughly investigated, it is unknown how Shigella first attaches to the epithelium. Previous literature suggests that Shigella utilizes adhesins that are induced by environmental signals
D L Scheusner et al.
Applied microbiology, 21(1), 46-49 (1971-01-01)
A population of Escherichia coli ML30 cells was exposed to a quaternary ammonium compound, and injury to the cells was measured by a comparison of counts on Trypticase Soy Agar and Violet Red Bile Agar. Substantial injury could not be
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Parasites & vectors, 6, 237-237 (2013-08-15)
Echinostomiasis is one of the major food-borne trematodiases and the species Echinostoma caproni serves as a useful model for trematocidal drug discovery. The current in vitro drug sensitivity assay uses adult E. caproni worms that are incubated with candidate drugs

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