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Roche

Adenosine Deaminase (ADA)

from calf intestine

Synonym(s):

ADA, Adenosine Deaminase

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About This Item

Enzyme Commission number:
3.5.4.4 (BRENDA, IUBMB)
UNSPSC Code:
12352204

biological source

bovine intestine

Quality Level

100

form

suspension

specific activity

~200 units/mg protein (At 25 °C with adenosine as the substrate.)

packaging

pkg of 1 mL (10 mg)

manufacturer/tradename

Roche

technique(s)

activity assay: suitable

color

white

optimum pH

7.0-7.4

solubility

water: miscible

suitability

suitable for chemiluminescence

NCBI accession no.

AAF91430

UniProt accession no.

P56658

application(s)

life science and biopharma

foreign activity

AMP-deaminase <0.01%
AP <0.01% (with pre incubation)
Guanase <0.01%
NP <0.01%

storage temp.

2-8°C

Gene Information

bovine ... ADA(280712)

General description

Adenosine aminohydrolase.
Adenosine deaminase (ADA) is a zinc metalloenzyme.

Application

Chemiluminescent adenosine assay.
Deamination of adenosine analogs to the corresponding inosine analogs
Adenosine deaminase (ADA) has been used in lipolysis assay along with other reagents.

Biochem/physiol Actions

Adenosine deaminase (ADA) is involved in the deamination of adenosine to inosine and 2′ deoxyadenosine to 2′-deoxyinosine.

Quality

Contaminants: <0.01% alkaline phosphatase, AMP-deaminase, guanase, and nucleoside phosphorylase each

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6. Small particles are normal. Before using stir up the precipitate and proceed.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cultured astrocytes do not release adenosine during hypoxic conditions.
Fujita T, et al.
Journal of Cerebral Blood Flow and Metabolism, 32(1), e1-e7 (2012)
Adenosine deaminase from calf intestinal mucosa.
C A ZITTLE
The Journal of biological chemistry, 166(2), 499-503 (1946-12-01)
D J Porter et al.
The Journal of biological chemistry, 268(4), 2480-2485 (1993-02-05)
The protein fluorescence of adenosine deaminase (ADA) was perturbed during the deamination of adenosine and four analogues of adenosine. The kinetics for the approach to the steady-state during turnover were monitored by fluorescence changes associated with formation of enzymatic intermediates.
Mi-Jeong Lee et al.
Methods in enzymology, 538, 49-65 (2014-02-18)
Obesity is reaching epidemic proportions so there is growing interest in the mechanisms that regulates adipose tissue development and function. Although murine adipose cell lines are useful for many mechanistic studies, primary human adipose stromal cells (ASCs), which can be
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