MAB2035
Anti-Chondroitin 6 Sulfate Antibody, clone MK-302
ascites fluid, clone MK-302, Chemicon®
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
MK-302, monoclonal
species reactivity
canine, sheep, rat, rabbit, mouse, bovine, human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
radioimmunoassay: suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... ACAN(176)
Specificity
Reacts with a wide range of cartilage proteoglycans after either chondroitinase (ABC or AC) or testicular hyaluronidase digestion. Native proteoglycans do not react with MAB2035. Antibody binding to epitope is successfully inhibited by disaccharides of chondroitin-6-sulfate. Recognizes the chondroitin-6-sulfate stubs (preferentially in clusters) of high density cartilage proteoglycans of different species. No cross-reactivity with dermatan sulfate, keratan sulfate or chondroitin-4-sulfate.
Immunogen
Chondroitinase ABC-digested adult human aggrecan.
Application
Anti-Chondroitin 6 Sulfate Antibody, clone MK-302 is an antibody against Chondroitin 6 Sulfate for use in ELISA, RIA & WB.
ELISA at 1:100-1:5,000
RIA at 1:100-1:200.
Chondroitinase ABC digestion prior to antibody reaction is required for antibody reactivity. {0.5U/mL in 100mM Tris-HCL, 30 minutes room temperature} A dramatic proteolytic digestion of the core protein (e.g. with papain or pronase) significantly reduces the antibody reactivity.
Optimal working dilutions must be determined by the end user.
RIA at 1:100-1:200.
Chondroitinase ABC digestion prior to antibody reaction is required for antibody reactivity. {0.5U/mL in 100mM Tris-HCL, 30 minutes room temperature} A dramatic proteolytic digestion of the core protein (e.g. with papain or pronase) significantly reduces the antibody reactivity.
Optimal working dilutions must be determined by the end user.
Physical form
Ascites liquid; no preservative.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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G D Nicodemus et al.
Journal of biomechanics, 41(7), 1528-1536 (2008-04-18)
Crosslinked poly(ethylene glycol) (PEG) hydrogels are attractive scaffolds for cartilage tissue engineering because of their ability to mimic the aqueous environment and mechanical properties of native cartilage. In this study, hydrogel crosslinking density was varied to study the influence of
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Idalis Villanueva et al.
Matrix biology : journal of the International Society for Matrix Biology, 29(1), 51-62 (2009-09-02)
This study aimed to elucidate the role of charge in mediating chondrocyte response to loading by employing synthetic 3D hydrogels. Specifically, neutral poly(ethylene glycol) (PEG) hydrogels were employed where negatively charged chondroitin sulfate (ChS), one of the main extracellular matrix
Robert E Hurst et al.
American journal of physiology. Renal physiology, 310(10), F1074-F1080 (2016-02-26)
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