AB3607

Anti-ASC Antibody

Chemicon^®, from rabbit

• Synonym(s): TMS1, CARD5, Apoptosis-associated Speck-like Protein containing a CARD
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 10

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): western blot: suitable
• NCBI accession no.: NM_013258.3, NM_145182.1, NM_145183.1
• UniProt accession no.: Q9ULZ3
• shipped in: wet ice
• target post-translational modification: unmodified

Description

General description

Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, Bcl10, Apaf-1, ARC, caspase-2 and caspase-9. A novel CARD domain containing protein has been identified in human and mouse and designated ASC and TMS1. Ectopic expression of ASC/TMS1 induced apoptosis through activation of caspase-9 and inhibited the survival of human breast cancer cells. Overexpression of ASC/TMS1 induced DNA fragmentation. ASC/TMS1 is expressed in a variety of human and mouse tissues.

Immunogen

Peptide corresponding to aa 182-195 (RESQSYLVEDLERS) of human ASC.

Application

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-ASC Antibody is validated for use in WB for the detection of ASC.

Western blot: 1:500

HL60 whole cell lysate can be used as a positive control.

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Detects a 25 kDa band by western blot, corresponding to ASC/TMS1.

Physical form

Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide.

Format: Purified

Preparation Note

Maintain at 4°C for up to 6 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• WGK: WGK 2
• Storage Class: 12 - Non Combustible Liquids