AB1837P
Anti-Tumor Necrosis Factor-α Antibody
Chemicon®, from rabbit
Synonym(s):
TNF-alpha
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... TNF(7124)
Specificity
Reacts with rat TNF-alpha
Immunogen
Highly purified (>98%) recombinant rat TNF-alpha
Application
Anti-Tumor Necrosis Factor-α Antibody is an antibody against Tumor Necrosis Factor-α for use in ELISA & WB.
ELISA: 0.5 μg/mL this dilution will allow detection (in direct ELISA) of
0.2-0.4 ng/well when rat TNF-α is used to coat the plate.
Western blot: 0.1 - 0.2 μg/mL, this dilution should offer detection limit of 1.5 - 3.0 ng/lane, under reducing or non-reducing conditions.
Optimal working dilutions must be determined by the end user.
0.2-0.4 ng/well when rat TNF-α is used to coat the plate.
Western blot: 0.1 - 0.2 μg/mL, this dilution should offer detection limit of 1.5 - 3.0 ng/lane, under reducing or non-reducing conditions.
Optimal working dilutions must be determined by the end user.
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
Linkage
Replaces: 04-1114
Physical form
Affinity Purified Immunoglobulin. Lyophilized from PBS pH 7.2. Contains no preservative. Reconstitute with sterile distilled water.
Storage and Stability
The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile water to a concentration of =0.2 mg/ml the antibody is stable for at least six weeks at 2-4°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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X J Fei et al.
Genetics and molecular research : GMR, 13(4), 10537-10544 (2014-12-17)
The dried roots of the plant Acanthopanax senticosus (AS) are used in traditional Oriental medicine and reportedly possess anti-inflammatory properties in vitro. However, whether AS has the same anti-inflammatory effect in vivo and the underlying mechanisms of this action remain
Fan Li et al.
Journal of neurochemistry, 106(5), 2093-2105 (2008-07-17)
Microglial cells are endowed with different potassium ion channels but their expression and specific functions have remained to be fully clarified. This study has shown Kv1.2 expression in the amoeboid microglia in the rat brain between 1 (P1) and 10
Clémence Guiraut et al.
International journal of molecular sciences, 17(12) (2016-11-30)
The medium-size intra-cranial arteries arising from the carotid bifurcation are prone to perinatal arterial ischemic strokes (PAIS). PAIS' physiopathology needs to be better understood to develop preventive and therapeutic interventions that are currently missing. We hypothesized that materno-fetal inflammation leads
Jiunn-Ming Sheen et al.
Frontiers in cellular neuroscience, 10, 167-167 (2016-07-23)
Rats subjected to bile duct ligation (BDL) exhibit increased systemic oxidative stress and brain dysfunction characteristic of hepatic encephalopathy (HE), including fatigue, neurotransmitter alterations, cognitive and motor impairment, and brain inflammation. The levels of tumor necrosis factor-alpha (TNF-α) and asymmetric
Devy Deliyanti et al.
Arteriosclerosis, thrombosis, and vascular biology, 34(9), 2033-2041 (2014-07-12)
Neovascularization and vaso-obliteration are vision-threatening events that develop by interactions between retinal vascular and glial cells. A high-salt diet is causal in cardiovascular and renal disease, which is linked to modulation of the renin-angiotensin-aldosterone system. However, it is not known
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