Skip to Content
Merck
All Photos(1)

Key Documents

662086

Sigma-Aldrich

UCH-L1 Inhibitor

The UCH-L1 Inhibitor, also referenced under CAS 668467-91-2, controls the biological activity of UCH-L1. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.

Synonym(s):

UCH-L1 Inhibitor, LDN-57444, Ubiquitin C-Terminal Hydrolase L1 Inhibitor, Ubiquitin C-Terminal Esterase L1 Inhibitor, Ubiquitin Thiolesterase L1 Inhibitor

Sign Into View Organizational & Contract Pricing


About This Item

Empirical Formula (Hill Notation):
C17H11Cl3N2O3
CAS Number:
Molecular Weight:
397.64
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.77

Quality Level

Assay

≥95% (HPLC)

form

solid

potency

0.88 μM IC50
0.40 μM Ki

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

orange

solubility

DMSO: 20 mg/mL

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C17H11Cl3N2O3/c1-9(23)25-21-16-13-7-12(19)3-5-15(13)22(17(16)24)8-10-6-11(18)2-4-14(10)20/h2-7H,8H2,1H3/b21-16-

InChI key

OPQRFPHLZZPCCH-PGMHBOJBSA-N

General description

A potent, reversible, competitive, and active site-directed inhibitor of ubiquitin C-terminal hydrolase L1 (UCH-L1) (Ki = 400 nM; IC50 = 880 nM). Exhibits ~28-fold greater selectivity for UCH-L1 compared to UCH-L3 (Cat. No. 662090). Shown to increase proliferation of the UCH-L1-expressing tumor cell line, SH-SY5Y, at a concentration of 5 µM. Useful tool for studying the roles of UCH-L1 in cancer, Parkinson′s disease, and other neurological disorders. Cell-permeability is reported to vary from cell type to cell type.
An isatin O-acyl oxime compound that acts as a potent, reversible, competitive, and active site-directed inhibitor of UCH-L1 (Ki = 0.40 µM; IC50 = 0.88 µM) with ~28-fold greater selectivity over UCH-L3 (Cat. No. 662090). Shown to increase proliferation of UCH-L1 expresssing tumor cell line SH-SY5Y at 5 µM. A useful tool for studying the roles of UCH-L1 in cancer, Parkinson’s disease, and other neurological disorders. Permeability may vary from cell type to cell type.

Biochem/physiol Actions

Cell permeable: no
Primary Target
UCH-L1
Product competes with ATP.
Reversible: yes

Packaging

Packaged under inert gas

Warning

Toxicity: Carcinogenic / Teratogenic (D)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Liu, Y., et al. 2003. Chem. Biol.10, 837.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Yize Li et al.
Cancer cell, 41(1), 139-163 (2022-12-24)
Clear cell renal cell carcinomas (ccRCCs) represent ∼75% of RCC cases and account for most RCC-associated deaths. Inter- and intratumoral heterogeneity (ITH) results in varying prognosis and treatment outcomes. To obtain the most comprehensive profile of ccRCC, we perform integrative

Related Content

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service