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Sigma-Aldrich

Anti-phospho-c-Jun (Ser73) Antibody

Upstate®, from rabbit

Synonym(s):

Activator protein 1, Jun activation domain binding protein, Proto-oncogene c-jun, V-jun avian sarcoma virus 17 oncogene homolog, enhancer-binding protein AP1, jun oncogene, v-jun sarcoma virus 17 oncogene homolog, v-jun sarcoma virus 17 oncogene homolog

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human

species reactivity (predicted by homology)

Xenopus (based on 100% sequence homology), chicken (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology), zebrafish (based on 100% sequence homology)

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NP_002219.1

UniProt accession no.

P05412

shipped in

wet ice

target post-translational modification

phosphorylation (pSer73)

Gene Information

human ... JUN(3725)
mouse ... Jun(16476)
rat ... Jun(24516)
zebrafish ... Jun(335916)

General description

c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements and appears to be a major downstream target of the SAPK/JNK signaling pathway. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced

signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun.

Specificity

This antibody detects phospho-c-Jun. May also detect phospho-JunD in properly treated cells since the sequence homology is identical (13/13).

Immunogen

KLH-conjugated linear peptide corresponding to the human Phospho-C-Jun (Ser73) near the N-terminus.

Application

Anti-phospho-c-Jun (Ser73) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-c-Jun (Ser73) also known as Activator protein 1 & has been validated in ICC, IP & WB.
Western Blot Analysis :
A previous lot of this antibody detected 10 ng of phosphorylated gst-Jun, but did not detect 10 ng of nonphosphorylated Jun, as reported by an independent laboratory.

Quality

Evaluated by Western Blotting in Anisomycin treated NIH/3T3 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Phospho-C-Jun (Ser73) in 10 µg of Anisomycin treated NIH/3T3 cell lysate.

Target description

~40 kDa

Linkage

Replaces: 04-213

Physical form

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% sodium azide.

Analysis Note

Control
Untreated and anisomycin-treated NIH/3T3 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M Repici et al.
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