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T4144

Sigma-Aldrich

Anti-Lectin, Triticum vulgaris antibody produced in rabbit

fractionated antiserum, lyophilized powder

Synonym(s):

Anti-WGA, Anti-Wheat germ lectin

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

fractionated antiserum

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

Triticum vulgaris

packaging

vial of 2 mL

technique(s)

indirect ELISA: 1:50,000

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Wheat germ lectin, also known as wheat germ agglutinin (WGA), has a molecular weight of 36 kDa. It shows affinity to 1,4-N-acetylglucosamine (GlcNAc) oligomers and is useful in studying surface components of C. neoformans.

Immunogen

Wheat Germ lectin (WGA)

Application

Anti-Lectin, Triticum vulgaris antibody produced in rabbit has been used in
  • immunolabeling experiments
  • immunohistochemistry
  • immunocytochemistry
Anti-Lectin, Triticum vulgaris antibody produced in rabbit has been used in immunohistochemistry and immunocytochemistry.

Biochem/physiol Actions

Wheat germ agglutinin is a lectin isolated from Triticum vulgaris that has affinity for chitin oligosaccharides, peptidoglycans and glycolipids via sialic acid residues. It is not specific for human blood groups and has been identified as antagonists of botulinum and tetanus toxins.

Target description

Lectins are highly specific carbohydrate binding proteins useful in carbohydrate and cell membrane studies, as well as cell agglutination and typing techniques.

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.2

Reconstitution

Reconstitute with 2 ml deionized water.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kazue Hashimoto-Torii et al.
IBRO reports, 5, 91-98 (2018-11-28)
Spinal cord and peripheral nerve injury results in extensive damage to the locally injured cells as well as distant cells that are functionally connected to them. Both primary and secondary damage can cause a broad range of clinical abnormalities, including
João Manuel Braz et al.
The Journal of comparative neurology, 507(6), 1990-2003 (2008-02-15)
Despite the evidence for a significant contribution of brainstem serotonergic (5HT) systems to the control of spinal cord "pain" transmission neurons, attention has turned recently to the influence of nonserotonergic neurons, including the facilitatory and inhibitory controls that originate from
João M Bráz et al.
The Journal of comparative neurology, 519(13), 2648-2657 (2011-04-13)
We recently developed a genetic transneuronal tracing approach that allows for the study of circuits that are altered by nerve injury. We generated transgenic (ZW-X) mice in which expression of a transneuronal tracer, wheat germ agglutinin (WGA), is induced in
Hélène Van de Vyver et al.
The American journal of pathology, 187(2), 268-279 (2017-01-16)
Staphylococcus aureus causes very serious infections of vascular grafts. Knowledge of the molecular mechanisms of this disease is largely lacking because of the absence of representable models. Therefore, the aim of this study was to set up a mouse model
Detection of local and remote cellular damage caused by spinal cord and peripheral nerve injury using a heat shock signaling reporter system
Hashimoto-Torii K, et al.
IBRO reports, 91-98 (2018)

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