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MAK451

Sigma-Aldrich

Glucose-6-Phosphate Dehydrogenase Assay Kit

Sufficient for 100 colorimetric tests

Synonym(s):

G6PD Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

Quality Level

input

plasma
serum
tissue
cell culture medium

detection method

colorimetric

relevant disease(s)

hematological disorder, anemia

storage temp.

−20°C

General description

Glucose-6-Phosphate Dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia.

Application

The Glucose-6-Phosphate Dehydrogenase Assay Kit may be used for:
  • Metabolism Research
  • Drug Discovery

Features and Benefits

  • Efficient and Precise: Requires only 20 μL of sample and a short 15-minute reaction time while detecting glucose-6-phosphate dehydrogenase levels across a wide range from 0.2 to 100 U/L, offering both convenience and sensitivity.
  • Compatibility with High-Throughput Systems: Effortlessly incorporate our kit into high-throughput handling systems, guaranteeing seamless integration and maintaining efficiency and accuracy in your laboratory operations.

Suitability

The kit is suitable for the quantitative determination of Glucose-6-Phosphate concentration and evaluation of drug effects on its metabolism in plasma, serum, tissue and culture media, etc.

Principle

The non-radioactive, colorimetric Glucose-6-Phosphate Dehydrogenase Assay Kit is based on the reduction of tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at A565 nm. The increase in absorbance at A565 nm is proportional to the enzyme activity.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Pictograms

Health hazard

Signal Word

Warning

Hazard Statements

Hazard Classifications

Muta. 2

Storage Class Code

12 - Non Combustible Liquids


Certificates of Analysis (COA)

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