I4777
Interferon-γ from mouse
≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym(s):
IFN-γ
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Recommended Products
biological source
mouse
Quality Level
recombinant
expressed in E. coli
Assay
≥98% (SDS-PAGE)
form
lyophilized powder
mol wt
protein 15.6 kDa
packaging
pkg of 0.1 mg
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
color
white
solubility
water: soluble 1.000 mL, clear, colorless
UniProt accession no.
storage temp.
−20°C
Gene Information
mouse ... Ifng(15978)
Application
Interferon-γ from mouse has been used:
- for in vitro stimulation of the BMDC (bone marrow derived dendritic cell), BMM (bone marrow-derived macrophages) and CD11c+ (CD11 antigen-like family member C) lung macrophages
- for stimulation of murine macrophages to induce the production of nitrite and nitrate
- to check CCAAT/enhancer binding protein β (C/EBPβ)-mediated changes in the expression of prostaglandin synthesis enzymes in neuroinflammation
Biochem/physiol Actions
Ifng (interferon-γ) plays an important role in innate and adaptive immune responses, and helps in defense against bacterial infections, dimorphic yeast and some viral infections.
Physical form
Lyophilized from a 0.2 μm filtered buffered solution. Package size based on protein content.
Analysis Note
The biological activity of recombinant mouse IFN-γ is measured by its ability to inhibit the proliferation of murine WEHI-279 cells.
related product
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Sergio Arias-Negrete et al.
Analytical biochemistry, 328(1), 14-21 (2004-04-15)
Preparation of a nitrate reductase lysate of Escherichia coli MC1061 to measure nitrate and nitrite in biologic fluids is described. To obtain the crude bacterial lysate containing nitrate reductase activity, E. coli MC1061 was subjected to 16-20 freeze-thawing cycles, from
S N Vogel et al.
Infection and immunity, 38(2), 681-685 (1982-11-01)
Silica has been used for many years as an agent which selectively alters macrophage functions and, as such, has been used to assess the role of macrophages in the immune response to a variety of microbial and chemically defined agents.
P W Gray et al.
Proceedings of the National Academy of Sciences of the United States of America, 80(19), 5842-5846 (1983-10-01)
The murine immune interferon (IFN-gamma) gene was cloned and expressed under control of the simian virus 40 early promoter in the monkey COS-1 cell line. A protein is secreted from these cells having the biological, antigenic, and biochemical characteristics of
Marco Straccia et al.
Glia, 61(10), 1607-1619 (2013-07-31)
The eicosanoid prostaglandin E2 (PGE2 ) plays important roles in neuroinflammation and it is produced by the sequential action of the enzymes cyclooxygenase-2 (COX-2) and prostaglandin E synthase (PTGES). The expression of both enzymes and the production of PGE2 are
Kiwon Park et al.
Journal of leukocyte biology, 88(6), 1081-1087 (2010-08-12)
The oxysterol-producing enzyme CH25H plays an important role in regulating lipid metabolism, gene expression, and immune activation. In vitro experiments using a panel of TLR agonists to activate BMDCs and macrophages demonstrated that Ch25h expression is induced rapidly, selectively, and
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service