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Key Documents

HA cl. 55

95040311, Reactivity: Anti-Measles Virus Haemagglutinin

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About This Item

UNSPSC Code:
41106514

biological source

Mouse x Mouse Hybridoma

ImmunoDon

BALB/c mouse spleen

Myeloma

Sp2/0 Ag-14

Reactivity

Anti-Measles Virus Haemagglutinin

technique(s)

cell culture | mammalian: suitable

isotype

IgG2b

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse BALB/c spleen x Mouse Sp2/0 Ag-14

Cell Line Description

The hybridoma cell line HA cl. 55 was produced by fusing myeloma cells with spleen cells derived from BALB/c mice immunised with purified measles virus. Immunofluorescence studies showed intracellular and membrane fluorescence with a diffuse pattern. The monoclonal antibody precipitates haemagglutinin polypeptide and neutralises measles virus infectivity. Biological assays showed haemagglutinin inhibition activity.

Immunogen

Measles virus (strain Hallé)

Application

Diagnosis, virus neutralisation.

Culture Medium

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 1-5x100,000 cells/ml; 5% CO2; 37°C.

Other Notes

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