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C3777

Sigma-Aldrich

Casein fluorescein isothiocyanate from bovine milk

Type II, essentially salt-free, lyophilized powder

Synonym(s):

FITC-casein

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About This Item

MDL number:
UNSPSC Code:
12352204
NACRES:
NA.32

type

Type II

form

essentially salt-free, lyophilized powder

extent of labeling

20-50 μg FITC per mg solid

solubility

water: 5 mg/mL, clear to hazy, yellow to orange

storage temp.

2-8°C

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General description

Casein is a major milk phospho-protein and constitutes about 80% of the total protein content in milk. Bovine milk is composed of four types of casein such as, αS1, αS2, β, and κ. Casein exists as micelles in milk.

Application

Casein fluorescein isothiocyanate from bovine milk has been used:
  • as a substrate for semi-quantitative analysis of protease in Arabidopsis cells
  • to determine the caseinolytic activity of secreted LasB (elastase), alkaline protease and protease IV
  • for determining the SpeB (cysteine protease) proteolytic activity in Streptococcus pyogenes cells

highly sensitive protease substrate

Biochem/physiol Actions

Casein micelle system is involved in the prevention of pathological calcification of mammary glands.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mark F Mabanglo et al.
Communications biology, 2, 410-410 (2019-11-23)
Bacterial ClpP is a highly conserved, cylindrical, self-compartmentalizing serine protease required for maintaining cellular proteostasis. Small molecule acyldepsipeptides (ADEPs) and activators of self-compartmentalized proteases 1 (ACP1s) cause dysregulation and activation of ClpP, leading to bacterial cell death, highlighting their potential
Swarming motility, secretion of type 3 effectors and biofilm formation phenotypes exhibited within a large cohort of Pseudomonas aeruginosa clinical isolates
Murray T S, et al.
Journal of Medical Microbiology, 59(Pt 5), 511-511 (2010)
Cationic antimicrobial peptides disrupt the Streptococcus pyogenes ExPortal
Vega LA and Caparon MG
Molecular Microbiology, 85(6), 1119-1132 (2012)
Casein and Whey Proteins in Human Health
Milk and Dairy Products as Functional Foods, 102(6), 94-146 (2014)
Thomas S Murray et al.
Journal of medical microbiology, 59(Pt 5), 511-520 (2010-01-23)
Pseudomonas aeruginosa is an opportunistic Gram-negative pathogen capable of acutely infecting or persistently colonizing susceptible hosts. P. aeruginosa colonizes surfaces in vitro by either biofilm formation or swarming motility. The choice of behaviour is influenced by the physical properties of

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