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H6378

Supelco

20α-Hydroxycholesterol

analytical standard

Synonym(s):

5-Cholestene-3β,20α-diol

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About This Item

Empirical Formula (Hill Notation):
C27H46O2
CAS Number:
516-72-3
Molecular Weight:
402.65
MDL number:
MFCD16661190
UNSPSC Code:
41116107
PubChem Substance ID:
329815230
NACRES:
NA.24

grade

analytical standard

Quality Level

200

Assay

≥98%

technique(s)

HPLC: suitable
gas chromatography (GC): suitable

application(s)

clinical testing

format

neat

SMILES string

CC(C)CCC[C@](C)(O)[C@H]1CC[C@H]2[C@@H]3CC=C4C[C@@H](O)CC[C@]4(C)[C@H]3CC[C@]12C

InChI

1S/C27H46O2/c1-18(2)7-6-14-27(5,29)24-11-10-22-21-9-8-19-17-20(28)12-15-25(19,3)23(21)13-16-26(22,24)4/h8,18,20-24,28-29H,6-7,9-17H2,1-5H3/t20-,21-,22-,23-,24-,25-,26-,27-/m0/s1

InChI key

MCKLJFJEQRYRQT-APGJSSKUSA-N

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Application

20α-Hydroxycholesterol has been used as a standard to determine the concentration of 20α-hydroxycholesterol, present in food samples using gas chromatography with an ion trap detector coupled with mass spectrometer (GC-IT-MS).
Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000276072
SLCD0481
SLBZ8817
SLBX0926
SLBV4688

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Cholesterol Sigma Grade, ≥99%

Sigma-Aldrich

C8667

Cholesterol

J W Laskey et al.
Toxicology and applied pharmacology, 108(2), 296-306 (1991-04-01)
In vivo assessment of toxicant action on Leydig cell function is subject to homeostatic mechanisms which make it difficult to determine whether any changes seen in serum testosterone (T) concentration are due to extragonadal endocrine alterations or to a direct
B C McNamara et al.
Molecular and cellular endocrinology, 73(2-3), 123-134 (1990-10-22)
Side-chain cleavage (SCC) of endogenous cholesterol in adrenal mitochondria isolated from ACTH-treated rats indicates that the size of the reactive cholesterol pool depends on the reducing precursor. At optimal concentrations of reductant, this pool was typically at least 2 times
Ying Yong Lin et al.
The Journal of steroid biochemistry and molecular biology, 85(1), 57-61 (2003-06-12)
The presence of 20(S)-hydroxycholesterol in rat brains and human placenta has been established using the gas chromatography/mass spectrometry (GC/MS) select ion monitoring (SIM) technique. Identification was ensured by three criteria: the specific retention time when the compound emerges from the
K Z Liu et al.
American heart journal, 123(2), 285-290 (1992-02-01)
Oxidized low-density lipoprotein (LDL) may play an important role in atherogenesis. The oxidative status of isolated LDL and very low-density lipoprotein (VLDL) were investigated in 23 patients with proven coronary disease and in 23 healthy asymptomatic control subjects. Oxidized cholesterol
J H Nielsen et al.
The Journal of dairy research, 62(1), 101-113 (1995-02-01)
A method for isolation, detection and quantification of cholesterol oxidation products based on solid phase extraction in combination with preparative HPLC and gas chromatography-mass spectrometry selected ion monitoring has been developed for dairy products. The isolation procedure had a high
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