76704

modified Tryptic Soy Broth- Dehydrated Culture Media

NutriSelect^® Plus, novobiocin 0.02 g/L, according to ISO 16654:2001, powder, selective for enterovirulent E. coli (EEC)

• Synonym(s): Modified Tryptic Soy Broth with Novobiocin, Modified Tryptic Soy Broth; EHEC enrichment broth, mTSB Broth with Novobiocin
• UNSPSC Code: 41171607
• NACRES: NA.85

Properties

• Agency: according to ISO 16654:2001
• Quality Level: 200
• sterility: non-sterile
• form: powder
• shelf life: limited, expiry date on the label
• composition: bile salts No. 3, 1.5 g/L ; di-potassium hydrogen phosphate, 4.0 g/L ; D(+)-glucose, 2.5 g/L ; novobiocin, 0.02 g/L ; peptone from casein, 17.0 g/L ; peptone from soymeal, 3.0 g/L ; sodium chloride, 5.0 g/L
• manufacturer/tradename: NutriSelect^® Plus
• packaging: pkg of -500 g
• storage condition: (Dry place, tightly sealed containers)
• technique(s): microbiological culture: suitable
• color: faint brown
• final pH: 7.3±0.2 (25 °C)
• application(s): agriculture; food and beverages; microbiology; pathogen testing
• shipped in: wet ice
• storage temp.: 2-8°C
• suitability: selective for Escherichia coli; selective for coliforms; selective for enterovirulent E. coli (EEC)

Description

General description

mTSB Broth with Novobiocin is suitable for the selective enrichment of enterohemorrhagic Escherichia coli (EHEC) in foods intended for human and animal consumption. The peptone provides amino acids and other complex nitrogenous substances. Glucose is a fermentable carbohydrate. Sodium chloride delivers osmotic balance. Bile salts inhibit the growth of Gram-positive accompanying bacterial flora. Di-potassium hydrogen phosphate is the buffering agent to stabilize the pH.mTSB is made selective for E. coli O157:H7 by adding bile salts in the media along with Novobiocin supplementation.

Application

mTSB Broth with Novobiocin is recommended for the selective enrichment of enterohemorrhagic E. coli (EHEC) in foods, especially meat and poultry products.The medium meets the requirements of the DIN Norm 10167 for the detection of E. coli serotype O157:H7 in foods. It is also recommended by the FDA-BAM as a method for the isolation of enterohemorrhagic E. coli (EHEC).

Preparation Note

Suspend 33 g in 1 litre of distilled water, autoclave at 121°C for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves