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Key Documents

MAB1406

Sigma-Aldrich

Anti-Thy-1.1 Antibody, clone OX-7

clone OX-7, Chemicon®, from mouse

Synonym(s):

CD90.1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

OX-7, monoclonal

species reactivity

rat

packaging

antibody small pack of 25 μg

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... THY1(7070)

Related Categories

Specificity

Directed against the Thy 1.1 antigenic determinant which is a monomorphic determinant within rat strains but polymorphic in the mouse (positive to AKR strain, negative to CVA and BALB/c). Thy-1 antigen is present on a variety of cell types including thymocytes, neuronal cells, T-lymphocytes (mouse), stem cells and immature B cells (rat) and connective tissue (Campbell, 1981). Thy-1 is a glycoprotein, 112 amino acids, which is homologous to immunoglobulin domains (Williams & Gagnon, 1982). MAB1406 can be coupled to sepharose 4-B and used to purify mouse Thy-1.1 and rat Thy-1 antigens (Campbell, 1981; Williams & Gagnon, 1982). MAB1406 can also be used to study the killing of Thy-1 tumor cells with antibody-toxin conjugates (Neville, 1982).

Immunogen

Rat Thy-1

Application

Anti-Thy-1.1 Antibody, clone OX-7 detects level of Thy-1.1 & has been published & validated for use in FC, IC, IH.
Flow Cytometry: 1:10-1:100. Use 10 μl per 10E6 thymocytes.

Immunohistochemistry on frozen sections.

Immunocytochemistry

Optimal working dilutions must be determined by end user.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

Quality

Tested

Physical form

Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Thymocytes, T-lymphocytes

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The membrane expression and gene promoter of the glycosylphosphatidylinositol (GPI)-anchored protein Thy1 have been widely used to examine the morphology and distribution of retinal ganglion cells in normal eyes and disease models. However, it is not known how adult mammalian

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