17-662
ChIPAb+ EZH2, clone AC22 - ChIP Validated Antibody and Primer Set
clone AC22, from mouse
Synonym(s):
Enhancer of zeste homolog 2, Lysine N-methyltransferase 6, enhancer of zeste 2, enhancer of zeste homolog 2, ENX-1
About This Item
Recommended Products
biological source
mouse
Quality Level
clone
AC22, monoclonal
species reactivity
mouse, rat, vertebrates, human
manufacturer/tradename
ChIPAb+
Upstate®
technique(s)
ChIP: suitable
western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
dry ice
General description
The ChIPAb+ EZH2, clone AC22 antibody/primer set includes the EZH2, clone AC22 antibody, a negative control antibody (purified mouse IgG), and qPCR primers which amplify a 135 bp region within the promoter of the human MYT-1 gene. The EZH2, clone AC22 antibody and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of EZH2-associated chromatin.
Specificity
Immunogen
Application
Representative lot data.
Sonicated chromatin prepared from HeLa S3 cells (4 X 106 cell equivalents per IP) was subjected to chromatin immunoprecipitation using 1 µg of either a normal mouse IgG or Anti-EZH2, clone AC22 antibody and the Magna ChIP G Kit (Cat. # 17-611).
Successful immunoprecipitation of EZH2-associated DNA fragments was verified by qPCR using GAPDH promoter (negative) and MYT-1 promoter (positive) primers (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna G ChIP (Cat. # 17-409) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Immunoprecipitation Analysis:
Representative lot data.
HeLa nuclear cell extract was Immunoprecipitated with either anti-EZH2, clone AC22 or mouse IgG and then resolved by electrophoresis, transferred to PVDF and probed with Cat. # 05-1319 anti-EZH2, clone BD43 (1:1000 dilution).
Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP (Cat. # AP124P) and a chemiluminescence detection system.
(Please see figures).
Lane 1: Hela nuclear extract immunoprecipitate with anti-EZH2, clone AC22.
Lane 2: Hela nuclear extract immunoprecipitate with IgG.
Epigenetics & Nuclear Function
Apoptosis & Cancer
Chromatin Biology
Packaging
Quality
Sonicated chromatin prepared from HeLa S3 cells (4 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either a normal mouse IgG or Anti-EZH2, clone AC22 antibody and the Magna ChIP® G Kit (Cat. # 17-611). Successful immunoprecipitation of EZH2-associated DNA fragments was verified by qPCR using Control Primers (Please see figures).
Please refer to the EZ-Magna ChIP G (Cat. # 17-409) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Target description
Physical form
Normal Mouse IgG. Two vials, each containing 25 μg purified mouse IgG in 25 μL storage buffer containing 0.1% sodium azide. Store at -20°C.
ChIP Primers MYT-1 promoter Region. One vial containing 75 μL of 5 μM of each primer specific for the promoter region of human MYT-1. Store at -20°C.
FOR: ACA AAG GCA GAT ACC CAA CG
REV: GCA GTT TCA AAA AGC CAT CC
Storage and Stability
Analysis Note
Includes negative control mouse IgG antibody and primers specific for of human MYT-1 promoter region.
Legal Information
Disclaimer
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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