07-476

Anti-p75NTR (Neurotrophin Receptor) Antibody

Upstate^®, from rabbit

• Synonym(s): CD271 antigen, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, NGF receptor, low affinity nerve growth factor receptor, nerve growth factor receptor, nerve growth factor receptor (TNFR superfamily, member 16), p75 IC
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, mouse, rat
• manufacturer/tradename: Upstate^®
• technique(s): immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_005028.4
• UniProt accession no.: P07174
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... NGFR(4804)

Description

General description

The Low Affinity Nerve Growth Factor Receptor (also called the LNGFR or p75 neurotrophin receptor) is one of the two receptor types for the neurotrophins, a family of protein growth factors which stimulate neuronal cells to survive and differentiate.
The neurotrophins are comprised of four proteins, all of which bind to the LNGFR: Nerve Growth Factor (NGF), Brain Derived Neurotrophic Factor (BDNF), Neurotrophin-3 (NT-3), and Neurotrophin-4 (NT-4).
Nerve growth factor, the prototypical growth factor, is a protein secreted by a neuron′s target. NGF is critical for the survival and maintenance of sympathetic and sensory neurons. NGF is released from the target cells, binds to and activates its high affinity receptor (TrkA), and is internalized into the responsive neuron. The NGF/TrkA complex is subsequently trafficked back to the cell body. This movement of NGF from axon tip to soma is thought to be involved in the long-distance signaling of neurons.

Specificity

Recognizes p75 neurotrophin receptor, MW ~75 kDa.

Immunogen

Epitope: IC domain

GST fusion protein corresponding to the intracellular domain (residues 274-425) of rat p75 neurotrophin receptor.

Application

Detect p75NTR (Neurotrophin Receptor) using this Anti-p75NTR (Neurotrophin Receptor) Antibody validated for use in IH, IP & WB.

Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate p75 neurotrophin receptor.

Immunohistochemistry:
This antibody has been reported by an independent laboratory to detect p75 neurotrophin receptor in rat brain sections. (Roux, P., 1999)

Research Category
Neuroscience

Research Sub Category
Neurochemistry & Neurotrophins

Quality

Evaluated by western blot in RIPA lysates from PC-12 cells.

Western Blot Analysis:
0.1-0.5 µg/mL of this antibody detected p75 neurotrophin receptor in RIPA lysates from PC-12 cells.

Target description

~75 kDa

Physical form

Format: Purified

Protein A purified

Purified rabbit polyclonal IgG in buffer containing 70% storage buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Brain tissue.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1