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Key Documents

G1007216

HPLC Analysis of Caffeine and Theophylline on SeQuant® ZIC-HILIC

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: 1.50448 Sequant ZIC-HILIC, PEEK, 150 x 4.6 mm, 5 μm, 200 Å (1.50448.0001)
mobile phase: [A] acetonitrile; [B] ammonium acetate; (90:10, A,B)
flow rate: 0.1 mL/min
pressure: 403 psi (2.8 MPa)
detector: 254 nm
injection: 5 μL

suitability

application for HPLC

application(s)

food and beverages

Legal Information

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Caffeine
    Theophylline

accessory

Product No.
Description
Pricing

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Hamilton® TLC syringes, 1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),

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Spectroline® E-Series UV lamp, output6 W, AC/DC input 120 V AC

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Hamilton® TLC syringes, 1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),

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Hamilton® TLC syringes, 1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),

coated tip

Z204153

Rectangular TLC developing tanks, complete, L × H × W 17.5 cm × 16.0 cm × 8.2 cm

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Spectroline® E-Series UV lamp, output4 W, AC/DC input 230 V AC

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Spectroline® E-Series UV lamp, output8 W, AC/DC input 230 V AC

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Latch-lid™ TLC developing chambers, For use with 10 cm × 10 cm plates

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Hamilton® TLC syringes, 1710, volume 100 μL, needle size 22 ga, needle L 51 mm (2 in.),

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Latch-lid™ TLC developing chambers, For use with 20 cm × 20 cm plates

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Spectroline® E-Series UV lamp, output4 W, AC/DC input 115 V AC

Z543241

Kimble® microcapillary pipettes

Z543268

Kimble® microcapillary pipettes

Z543276

Kimble® microcapillary pipettes, capacity 20 μL, accuracy: ±0.5%

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Reagent sprayer for TLC plates, volume 250 mL, Bottle-Type

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