Skip to Content
Merck
All Photos(1)

Documents

G005540

HPLC Analysis of Dermorphin in Horse Plasma on Ascentis® Express F5 after SPE with HybridSPE®

application for HPLC, application for SPE

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

technique(s)

HPLC: suitable
solid phase extraction (SPE): suitable

test parameters

sample preparation: SPE (Solid Phase Extraction)
sample/matrix: horse plasma spiked at 10 μg/mL
SPE tube/cartridge: [TM"HybridSPE"]-Phospholipid Plate, 50mg/96-Well (575656-U)
sample addition: 3:1, (1% formic acid in acetonitrile); plasma
column: Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)
mobile phase: [A] acetonitrile [B] 20 mM ammonium formate; (90:10, A:B); pH 4.5 with formic acid, after mixing A & B
flow rate: 0.4 mL/min
pressure: 1305 psi (90 bar)
column temp.: 35 °C
detector: MS, ESI(+), TIC, m/z 100-1000
injection: 1 μL

suitability

application for HPLC
application for SPE

application(s)

forensics and toxicology

Analysis Note

Dermorphin is a natural heptapeptide opioid. Because of poor recovery from horse plasma following protein precipitation, alternate methods of extraction are sought. While the HybridSPE protocol successfully removes proteins and phospholipids, dermorphin solubility in plasma remains a limitation. Dermorphin is effectively retained on the pentafluorophenyl phase under aqueous normal phase conditions.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany

analytical column

Product No.
Description
Pricing

mobile phase component

Product No.
Description
Pricing

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service