59141
SUPELCOSIL™ LC-ABZ (5 µm) HPLC Columns
L × I.D. 5 cm × 4.6 mm, HPLC Column
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Product Name
SUPELCOSIL™ LC-ABZ HPLC Column, 5 μm particle size, L × I.D. 5 cm × 4.6 mm
Agency
suitable for USP L60
Quality Level
feature
endcapped
manufacturer/tradename
SUPELCOSIL™
extent of labeling
12.0% carbon loading
parameter
≤70 °C temp. limit
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
L × I.D.
5 cm × 4.6 mm
surface area
170 m2/g
surface coverage
surface coverage 3.4 μmol/m2
matrix
silica gel, spherical particle platform
matrix active group
amide, alkyl phase
particle size
5 μm
pore size
120 Å
pH range
2-7.5
application(s)
food and beverages
separation technique
reversed phase
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General description
SUPELCOSIL LC-ABZ phase has a unique deactivation technology which provides excellent reversed-phase performance for basic compounds, as well as those that are acidic, polar neutral, and non-polar. Silanol suppressing practices such as using competing amines, operating at high ionic strength, and adjusting mobile phases to pH extremes are typically not required to obtain good peak shape on the LC-ABZ phase.
Features and Benefits
• For acids, bases, zwitterions
• Increased polar retention relative to standard reversed-phases
• Unique selectivity
• Silanol deactivation
• Peak shape, efficiency, and retention are comparable to C8 phases for non-polar analytes
• Increased polar retention relative to standard reversed-phases
• Unique selectivity
• Silanol deactivation
• Peak shape, efficiency, and retention are comparable to C8 phases for non-polar analytes
Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
guard cartridge
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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J G Ufkes et al.
Pharmacy world & science : PWS, 20(2), 83-87 (1998-05-19)
To study the pharmacokinetics of dextromoramide in long-term opiate addicts on methadone maintenance therapy (MMT) a reverse-phase HPLC technique was developed to monitor dextromoramide and methadone concentrations in plasma simultaneously. After liquid-liquid extraction from plasma, dextromoramide and methadone were determined
Neil C Dias et al.
The Analyst, 128(5), 427-433 (2003-06-07)
The solvation parameter model is used to identify suitable chromatographic models for estimating the octanol-water partition coefficient for neutral compounds of varied structure by reversed-phase liquid chromatography. The stationary phase Supelcosil LC-ABZ with methanol-water mobile phases affords a series of
K Valkó et al.
Journal of pharmaceutical and biomedical analysis, 11(4-5), 361-366 (1993-04-01)
S-Adenosyl-L-methionine (SAM) and its metabolites S-adenosyl-L-homocysteine (SAH) and methyl-thioadenosine (MTA) are endogenous compounds that are heavily involved in a variety of biochemical processes, and have therefore been the target for several assays in body fluids and tissues. Reversed-phase chromatographic behaviour
D Korakas et al.
Journal of pharmaceutical and biomedical analysis, 12(9), 1141-1145 (1994-09-01)
A series of biologically active peptides and their conjugates with lipidic amino acids were investigated by systematic change of the mobile phase composition using traditional octadecylsilica stationary phase and the newly developed Supelcosil LC-ABZ column. The mobile phases contained various
J A López et al.
Journal of chromatography. A, 870(1-2), 105-114 (2000-03-18)
A high-performance liquid chromatographic method with electrochemical detection has been developed for the determination of six 1,4-dihydropyridines: nifedipine, nimodipine, nisoldipine, nicardipine, felodipine and lacidipine. The chromatographic separation was performed using a Supelcosil LC-ABZ+Plus C18 column. A mobile phase of methanol-water
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