15023AST

Astec^® CHIROBIOTIC^® V2 Chiral (5 μm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

• Synonym(s): CHIROBIOTIC V2 Chiral Chromatography Column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• product name: Astec^® CHIROBIOTIC^® V2 Chiral HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
• material: stainless steel column
• Agency: suitable for USP L88
• description: HPLC column
• product line: Astec^®
• packaging: pkg of 1 ea
• manufacturer/tradename: Astec^®
• parameter: 0-45 °C temperature; 241 bar pressure (3500 psi)
• technique(s): HPLC: suitable; LC/MS: suitable
• L × I.D.: 15 cm × 4.6 mm
• matrix: High-purity silica gel particle platform; fully porous particle
• matrix active group: vancomycin phase
• particle size: 5 μm
• pore size: 200 Å
• operating pH range: 3.5-7.0
• separation technique: chiral

Description

General description

Neutral molecules, amides, acids, esters and amines show considerable enantioselectivity on these vancomycin-based CSPs. A wide variety of secondary and tertiary amines have been separated on the CHIROBIOTIC^® V in the polar ionic mode. The CHIROBIOTIC^® V has demonstrated many of the separation characteristics of protein-based stationary phases, but with exceptional stability and much higher sample capacity. Some chiral analytes have been resolved that have not been reported separated on any other chiral stationary phase. CHIROBIOTIC^® V and V2 differ in their bonding chemistry the pore size of the support particle, giving them different selectivity and preparative capacity.

• Bonded phase: Vancomycin
• Operating pH range: 3.5 - 7.0
• Particle diameter: 5, 10 or 16 μm
• Pore size: 100 Å (CHIROBIOTIC^® V) or 200 Å (CHIROBIOTIC^® V2)

CHIROBIOTIC FAQs
CHIROBIOTIC Reference Bibliography
Chiral Product Literature

Application

• Development and validation of an UFLC-MS/MS method for enantioselectivity determination of d,l-thero-methylphenidate, d,l-thero-ethylphenidate and d,l-thero-ritalinic acid in rat plasma and its application to pharmacokinetic study.: This research showcases the Astec^® CHIROBIOTIC^® V2 Chiral HPLC Column′s capacity for precise enantioselective analysis in pharmacokinetic studies, specifically for psychoactive substances. It highlights the column′s effectiveness in separating and quantifying chiral drugs and their metabolites in plasma, providing valuable insights for drug development and therapeutic monitoring (Zhang et al., 2016).


• New high-performance liquid chromatography method for the determination of (R)-warfarin and (S)-warfarin using chiral separation on a glycopeptide-based stationary phase.: This study illustrates the use of the Astec^® CHIROBIOTIC^® V2 Chiral HPLC Column for the chiral separation of warfarin enantiomers. The methodology developed provides a robust tool for the analysis of anticoagulant drugs, aiding in the assessment of their pharmacokinetics and optimizing dosage for therapeutic efficacy (Malakova et al., 2009).

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Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany

CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable