ZMS1024

Anti-Iba1/ AIF1 Antibody, clone 20A12.1 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Allograft inflammatory factor 1, Ionized calcium-binding adapter molecule 1, Protein G1
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: 20A12.1, recombinant monoclonal, monoclonal
• Application: ICC, IF, IHC (p), WB
• Citations: -

Properties

• biological source: mouse
• Quality Segment: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 20A12.1, recombinant monoclonal, monoclonal
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 16.7 kDa, observed mol wt ~17 kDa
• species reactivity: mouse, rat, human
• packaging: antibody small pack of 25 μL
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Sustainability features
• technique(s): immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
• isotype: IgG1
• UniProt accession no.: P55008
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... AIF1(199)

Description

General description

This product aligns with one or more of the 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Allograft inflammatory factor 1 (UniProt: P55008; also known as AIF-1, Ionized calcium-binding adapter molecule 1, Protein G1, Iba1) is encoded by the AIF1 (also known as G1, IBA1) gene (Gene ID: 199) in human. Iba1/AIF1 is a highly conserved cytoplasmic, calcium binding, inflammation responsive scaffold protein that is detected in T-lymphocytes and peripheral blood mononuclear cells. It has also been shown to associate with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis. It is not present in normal arteries, but its expression is induced in vascular smooth muscle cells in response to arterial injury where it enhances growth and promote proliferation in vascular smooth muscle cells through deregulation of the cell cycle by shortening the cell cycle and altering the expression of cyclins. AIF1 is also found in activated macrophages where its expression is augmented by cytokines, particularly interferon gamma. It is also considered as a marker for activated macrophages. Serum AIF-1 levels have been correlated with albuminuria and eGFR in type 2 diabetic subjects and serves as a marker for diabetic nephropathy. Three isoforms of AIF1/IBA1 have been described that are produced by alternative splicing. This clone will react with isoforms 1 and 2 that contain this immunogen sequence. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Fukui, M., et al. (2012). Diabetes Res. Clin. Pract. 98(1); 146-150; Autieri, MV., and Carbone, CM (2001). Arterioscler. Thromb. Vasc. Biol. 21(9);1421-1426).

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Immunogen

KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal region of human Allograft Inflammatory Factor 1 (AIF1).

Application

Anti-Iba1/ AIF1, clone 20A12.1 ZooMAb, Cat. No. ZMS1024, is a recombinant Mouse monoclonal antibody that targets Iba/AIF1 and is tested used in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Iba1/AIF1 in human spleen, rat spleen, and U937 cell lysates.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Iba1/AIF1 in human cerebellum tissue sections.

Immunocytochemistry Analysis: A 1:10 dilution from a representative lot detected Iba1/AIF1 in U-937 and THP-1 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Iba1/ AIF1 in human tonsil and human cerebellum tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Biochem/physiol Actions

Clone 20A12.1 is a ZooMAb mouse recombinant monoclonal antibody that specifically detects Allograft inflammatory factor 1 (Iba1/AIF1).

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Preparation Note

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 1