X3254
Xylanase 1, thermostable
recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Synonym(s):
Xylanase , thermostable
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About This Item
CAS Number:
UNSPSC Code:
12352204
Recommended Products
recombinant
expressed in E. coli
Assay
≥90% (SDS-PAGE)
form
liquid
specific activity
≥40 units/mg protein
mol wt
45 kDa
concentration
≥20 mg protein/mL (Bradford)
shipped in
wet ice
storage temp.
2-8°C
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Biochem/physiol Actions
Xylanase is a member of a family of glycoside hydrolases responsible for the breakdown of xylan in plants by cleaving the β 1,4 backbone.
Unit Definition
One unit will produce 1 μmole of reducing sugar (measured as xylose) from xylan per minute at pH 5.8 at 70 °C.
Physical form
Supplied as a solution in 50 mM Tris-HCl, pH 7.5, 100 mM NaCl, and 25% glycerol.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Tony Collins et al.
FEMS microbiology reviews, 29(1), 3-23 (2005-01-18)
Xylanases are hydrolytic enzymes which randomly cleave the beta 1,4 backbone of the complex plant cell wall polysaccharide xylan. Diverse forms of these enzymes exist, displaying varying folds, mechanisms of action, substrate specificities, hydrolytic activities (yields, rates and products) and
Ming-Qi Liu et al.
Protein expression and purification, 88(1), 85-92 (2012-12-19)
The xylan binding domain (XBD) and linker sequences (LS) from thermostable and thermophilic Thermomonospora fusca xylanase A (TfxA) was fused to the carboxyl-terminus of a family 11 hybrid xylanase ATx. The constructed chimera (ATxX) was successfully expressed in Pichia pastoris
Dong-Ju Park et al.
Journal of microbiology and biotechnology, 23(3), 397-404 (2013-03-07)
Paenibacillus xylanilyticus KJ-03 was isolated from soil samples obtained from a field with Amorphophallus konjac plants. A gene encoding xylanase was isolated from KJ-03 and cloned using a fosmid library. The xynA gene encodes xylanase; it consists of 1,035 bp
Digvijay Verma et al.
PloS one, 8(1), e52459-e52459 (2013-02-06)
The alkalistable and thermostable xylanases are in high demand for pulp bleaching in paper industry and generating xylooligosaccharides by hydrolyzing xylan component of agro-residues. The compost-soil samples, one of the hot environments, are expected to be a rich source of
Rou Min et al.
Wei sheng wu xue bao = Acta microbiologica Sinica, 53(4), 346-352 (2013-07-19)
To reveal the correlation between thermostability of xylanase EvXyn11(TS) and its N-terminal disulfide bridge, an EvXyn11(TS)-encoding gene (Syxyn11) was synthesized and subjected to site-directed mutagenesis. Multiple homology alignment of protein primary structures between the EvXyn11(TS) and several GH family 11
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