T2402

Anti-Mouse IgG (whole molecule)−TRITC antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Rabbit Anti-Mouse IgG (whole molecule)−Tetramethylrhodamine isothiocyanate
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162646
• Conjugate: TRITC conjugate
• Clone: polyclonal
• Application: IF (d), IHC (p)
• Citations: 25

Properties

• biological source: rabbit
• Quality Segment: 200
• conjugate: TRITC conjugate
• antibody form: IgG fraction of antiserum
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• storage condition: protect from light
• technique(s): direct immunofluorescence: 1:64, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Immunoglobulins (Igs) belongs immunoglobulin super-family. There are five different types of Immunoglobulins. Each immunoglobulin has two heavy (H) and two light (L), held together by disulphide linkages. Each light comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. IgG is abundant immunoglobulin in human serum. The four classes of IgG can be IgG1, IgG2, IgG3, and IgG4.

Immunogen

Normal mouse serum

Application

Anti-Mouse IgG (whole molecule)-TRITC antibody is suitable for use in direct immunofluorescence (1:64), immunohistochemistry (1:200 dilution in formalin-fixed, paraffin-embedded sections), Ouchterlony double diffusion and electrophoresis techniques.

Biochem/physiol Actions

Anti-Mouse IgG (whole molecule)-TRITC antibody is specific for mouse IgG.

Digestion of IgG by papain results in generation of fragment antigen binding (Fab) comprising one complete L chain and a variable and CH1 region of H chain. Pepsin digestion of IgG results in fragment crystallizable (fc), comprises the H chain constant region.

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice .

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)