T0438
Monoclonal Anti-Transforming Growth Factor-β1 antibody produced in mouse
clone 9016, purified immunoglobulin, lyophilized powder
Synonym(s):
Anti-TGF-β1, CEDLAP, DPD1, latency-associated peptide
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About This Item
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
9016, monoclonal
form
lyophilized powder
species reactivity
mouse, human, rat
technique(s)
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
neutralization: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
application(s)
research pathology
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... TGFB1(7040)
mouse ... Tgfb1(21803)
rat ... Tgfb1(59086)
General description
Transforming Growth Factor-β1 (TGFβ1) is mapped to human chromosome 19q13.2.
Transforming growth factor-β (TGF-β) belongs to the TGF-β superfamily of secreted proteins. TGF-β 1 exists as three isoforms TGF-β 1, TGF-β 2 and TGF-β 3. The TGF-β family is involved in a variety of signaling pathways that involve serine/threonine protein kinases. TGF-β acts through binding to three transmembrane receptors in mammals, type I, type II and type III receptors. The important pathways regulated by TGF-β are the SMAD, BMP, MAPK and PI3K/Akt pathways. TGF-β 1 is involved in cell and tissue differentiation, proliferation, inflammation, migration of monocytes, neutrophils and chemotaxis. Deregulation of TGF-β signaling has been implicated in many inflammation and autoimmune diseases. Monoclonal Anti-Transforming Growth Factor-β 1 antibody specifically reacts with human, mouse and rat TGF-β 1. The antibody shows less than 2% cross reactivity with TGF-β3 and TGF-β5 in ELISA.
Specificity
By capture ELISA, <2% cross-reactivity is observed with TGF-β3 and TGF-β5.
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant TGF- beta 1 and latent TGF- beta 1
Application
Recommended concentrations:
Western Blot 1 ug/mL (under non-reducing conditions only)
Immunohistochemistry 3-25 μg/mL
Flow Cytometry 2.5 μg/106 cells
ELISA capture 2-8 μg/mL
ELISA detection 0.1-0.4 μg/mL
Neutrilization 0.3-1.0 μg/mL
Western Blot 1 ug/mL (under non-reducing conditions only)
Immunohistochemistry 3-25 μg/mL
Flow Cytometry 2.5 μg/106 cells
ELISA capture 2-8 μg/mL
ELISA detection 0.1-0.4 μg/mL
Neutrilization 0.3-1.0 μg/mL
Monoclonal Anti-Transforming Growth Factor-β1 antibody produced in mouse has been used in:
- immunohistochemical detection of TGFβ1 in smooth muscle(2) and liver sections
- western blotting and ELISA of brain specimens
- immunoblot detection of TGFβ1 in macrophages
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Other Notes
Protein A or G purified from hybridoma culture supernatant.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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