SRP6327

CD274 from mouse

recombinant, expressed in E. coli, ≥85% (SDS-PAGE)

• Synonym(s): A530045L16Rik, B7h1, CD274 antigen, Pdcd1l1, Pdcd1lg1, Pdl1
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: mouse
• recombinant: expressed in E. coli
• Assay: ≥85% (SDS-PAGE)
• form: liquid
• mol wt: 27.6 kDa (246 aa, 19-239 aa + His tag)
• packaging: pkg of 100 μg
• concentration: 1 mg/mL
• impurities: <1 EU/μg endotoxin (LAL test)
• UniProt accession no.: Q9EP73
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: mouse ... CD274(60533)

Description

General description

Programmed cell death 1 ligand 1 (PD-L1), which is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules. PD-L1 is a cell surface immunoglobulin with two Ig-like domains within the extracellular region and a short cytoplasmic domain. The protein is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. Recombinant mouse CD274 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Biochem/physiol Actions

Programmed cell death 1 ligand 1 (PD-L1) is involved in the regulation of cellular and humoral immune responses. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after lipopolysaccharide (LPS) and interferon-γ (IFNG) activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 is involved in the co-stimulatory signal, essential for T-cell proliferation and production of interleukin-10 (IL10) and IFNG, in an interleukin-2-dependent and a programmed cell death 1 (PDCD1)-independent manner.

Physical form

1 mg/mL in 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl and 10% glycerol.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable