SRP4136
IL-3 from rat
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)
Synonym(s):
HCGF, IL-3, Interleukin-3, MCGF (Mast cell growth factor), MGC79398, Multi-CSF, P-cell stimulation factor
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About This Item
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biological source
rat
recombinant
expressed in E. coli
Assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
potency
<10 ng/mL ED50
mol wt
~15.8kDa
packaging
pkg of 10 μg
impurities
endotoxin, tested
NCBI accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
rat ... Il3(24495)
General description
Interleukin-3 (IL-3) is a species-specific colony-stimulating factor that stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. Recombinant rat IL-3 is a 15.8 kDa protein.
Physical form
Sterile filtered and lyophilized from 5 mM sodium citrate, pH 4.0.
Reconstitution
Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute in water to a concentration of 0.1-1.0 μg/μL. The solution can be diluted into other aqueous buffers.
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Weidong Yu et al.
Carcinogenesis, 29(9), 1717-1724 (2008-05-06)
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Molecular therapy. Nucleic acids, 3, e170-e170 (2014-06-25)
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Reciprocal interactions between tumor cells and their microenvironment vitally impact tumor progression. In this study, we show that GM-CSF produced by primary breast tumor cells induced the activation of plasmacytoid predendritic cells (pDC), a cell type critical to anti-viral immunity.
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Treatment with erythropoietin (EPO) in several cancers is associated with decreased survival due to cancer progression. Due to the major importance of telomerase in cancer biology we hypothesized that some of these effects may be mediated through EPO effect on
Attila Szabo et al.
Immunology and cell biology, 92(8), 671-678 (2014-05-21)
Plasmacytoid dendritic cells (pDCs) are professional type I interferon (IFN)-producing cells that play an essential role in antiviral immunity. In many cell types, detection of intracellular pathogens is mostly dependent on endosomal Toll-like receptors (TLRs) and cytosolic sensors, such as
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