SRP2119

RNA Polymerase II, RPB12 subunit, GST tagged human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

• Synonym(s): ABC10-alpha, RPABC4, RPB10alpha, RPB12, RPB7.0, hRPB7.0, hsRPB10a
• UNSPSC Code: 12352202
• NACRES: NA.26

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥80% (SDS-PAGE)
• form: frozen liquid
• mol wt: ~33 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: 100 μg/mL
• color: clear colorless
• NCBI accession no.: NM_005034
• UniProt accession no.: P53803
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... POLR2K(5440)

Description

General description

POLR2K (RNA polymerase II subunit K) is located on human chromosome 8q22. Rpb12 is the eukaryotic subunit that is also called as ABC10α. Rpb12 is the general subunit of all three eukaryotic RNA polymerases.

Biochem/physiol Actions

RPB12 (also called RPB10 ) is shared between all three RNA polymerases and is homologous to the archaeal P subunit of RNA polymerase. Like RPB10, it is essential for yeast cell viability. RPB12 is also a zinc-binding protein with a canonical tetra-coordinating zinc motif but binds zinc in vitro much less efficiently than the RPB10 subunit. It has been shown to interact with both RPB1 and RPB2 in vitro and the human subunit can functionally substitute for its yeast counterpart.

RPB12/RPC10 (RNA polymerase II subunit K) is required for growth in Saccharomyces cerevisiae. The carboxy-terminal domain (CTD) of RNAP II plays a major role in recruiting many of these activities to the transcriptional machinery.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable