SRP0124
CoREST human
recombinant, expressed in E. coli, ≥60% (SDS-PAGE)
Synonym(s):
RCOR1, REST corepressor 1
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
biological source
human
recombinant
expressed in E. coli
Assay
≥60% (SDS-PAGE)
form
aqueous solution
mol wt
20 kDa
packaging
pkg of 100 μg
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... RCOR1(23186)
General description
CoREST (also referred to as RCOR1 (REST corepressor 1)) mRNA is ubiquitously expressed in all tissues. It is a corepressor of the RE1-silencing transcription factor REST. In nonneuronal cells, the transcription factor binds to RE1 neuron-restrictive silencer elements and suppresses gene expression. CoREST is a component of the BRAF35 (BRCA2-associated factor 35)–histone deacetylase complex. The RCOR1 gene is mapped to human chromosome 14q32.
Human recombinant CoREST, GenBank Accession No. NM_015156), amino acids 305-end, with N-terminal His tag, MW = 20kDa, expressed in Escherichia coli expression system.
Human recombinant CoREST, GenBank Accession No. NM_015156), amino acids 305-end, with N-terminal His tag, MW = 20kDa, expressed in Escherichia coli expression system.
Application
Useful for the stimulation of LSD1 enzyme activity
Biochem/physiol Actions
CoREST associates with REST (RE1-silencing transcription factor), the histone deacetylases 1 and 2 (HDAC1/2), and LSD1 (lysine-specific histone demethylase 1A). This complex is involved in chromatin remodeling for changing gene expression. The LSD1/CoREST/REST compressor complex is also required for the expression of Herpes Simplex virus genes. LSD1 along with CoREST is responsible for the oxidative demethylation of the H3 histone (mono- and di-methylated Lysine4 residue). Deletions in the CoREST gene results in unfavorable clinical outcomes in DLBCL (diffuse large B-cell lymphoma) patients. It might also participate in the epigenetic control of blood cell development.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
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CoREST: a functional corepressor required for regulation of neural-specific gene expression.
Andres ME, et al.
Proceedings of the National Academy of Sciences of the USA, 96, 9873-9873 (1999)
An RCOR1 loss-associated gene expression signature identifies a prognostically significant DLBCL subgroup.
Chan FC, et al.
Blood, 125, 959-959 (2015)
Julie H Coleman et al.
The Journal of comparative neurology, 525(16), 3391-3413 (2017-06-10)
Neurons in the olfactory epithelium (OE) each express a single dominant olfactory receptor (OR) allele from among roughly 1,000 different OR genes. While monogenic and monoallelic OR expression has been appreciated for over two decades, regulators of this process are
Array comparative genomic hybridization and sequencing of 23 genes in 80 patients with myelofibrosis at chronic or acute phase.
Brecqueville M, et al.
Haematologica, 99, 37-37 (2014)
The Corepressor Rcor1 Is Essential for Normal Myeloerythroid Lineage Differentiation.
Yao H, et al.
Stem Cells, 33, 3304-3304 (2015)
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