SAB5202071

Monoclonal Anti-beta 4 Spectrin-Horseradish Peroxidase antibody produced in mouse

clone S393-2, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-SPTBN4
• UNSPSC Code: 12352203
• NACRES: NA.45

Properties

• biological source: mouse
• conjugate: peroxidase conjugate
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: S393-2; monoclonal
• description: clone S393-2, purified immunoglobulin, buffered aqueous solution; target molecular weight: 200 kDa
• form: buffered aqueous solution
• species reactivity: human (Tested), rat (Predicted), African green monkey (Tested), mouse (Predicted)
• packaging: pkg of 100 μg
• storage condition: avoid repeated freeze/thaw cycles; protect from light
• concentration: 1.0 mg/mL
• technique(s): immunofluorescence: 1:100; western blot: 1:1000
• isotype: IgG1
• UniProt accession no.: Q9H254
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SPTBN4(57731)

Description

Immunogen

Immunogen Description: Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin

Immunogen Region: C-terminal

Immunogen Species: Human

Immunogen type: Fusion protein

Application

Western blotting
Dilution 1:1000
Tissue/Cell Type Used: monkey COS-Beta-4-Spectrin-His

Immunofluorescence
Dilution 1:100
Tissue/Cell Type Used: SK-N-BE

Physical form

50% glycerol, 0.1% sodium azide

PBS pH 7.4

Preparation Note

Derived from animal serum

Storage and Stability

Protect from light; avoid repeated freeze-thaw cycles. Store at -20 °C long-term, 2-8 °C short-term.

Other Notes

Additional Product Details:
Does not cross react with other Beta-spectrins
Lot Expiration/Retest Date: Expires 1 year from purchase

Target/Gene Description: Spectrin is the major constituent of the cytoskeletal network underlying the erthrocyte plasma membrane and determines cell shape, arrangement of transmembrane proteins and organization of organelles. It is expressed at very low levels in many tissues, with the strongest expression in the cerebellum, spinal cord, stomach, pituitary gland, liver, pancreas, salivary gland, kidney, bladder and heart . Spectrin beta 4 is a non-erythrocyte member, and is expressed in the brain and pancreatic islets and localisted to the nuclear matrix, cytoplasmic vesicles and PML nuclear bodies. Beta4 spectrins are also essential for membrane stability and the molecular organization of the nodes of Ranvier .
Purification method: Protein G purified
Product type: Primary antibody
Antibody structure: Whole Antibody
Protein ID: NP_066022.2

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable