SAB4200578

Anti-EPS15 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-AF-1P, Anti-AF1P, Anti-MLLT5
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~135 kDa
• species reactivity: mouse, human, rat
• concentration: ~1.0 mg/mL
• technique(s): immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin-embedded human prostate carcinoma; indirect immunofluorescence: 5-10 μg/mL using HS68 cells; western blot: 1-2 μg/mL using extracts of Jurkat, NIH3T3 and PC12 cells
• UniProt accession no.: P42566
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... EPS15(2060) ; mouse ... Eps15(13858) ; rat ... Eps15(313474)

Description

General description

EGFR pathway substrate 15 (Eps15) is an endocytic adaptor protein. It contains three N-terminal conserved EH (epsl5 homology) protein-protein interaction domains, a central coiled-coil region, and a C-terminal domain containing the adapter protein (AP)-2 and AP-1 binding domains. The C-terminal domain also contains two ubiquitin interaction motifs (UIMs), required for Eps15 binding to ubiquitin. Eps15 colocalizes with markers of the plasma membrane clathrin-coated pits and vesicles.

Immunogen

synthetic peptide corresponding to an internal sequence of human EPS15 isoform A (GeneID: 2060), conjugated to KLH. The corresponding sequence is identical in human EPS15 isoform B, and in rat and mouse EPS15.

Application

Anti-EPS15 antibody produced in rabbit has been used in several immunochemical techniques including:

• immunoblotting
• immunofluorescence
• immunohistochemistry

Biochem/physiol Actions

Epidermal growth factor (EGFR) pathway substrate 15 (Eps15), that plays a central role in the clathrin mediated internalization and degradation of EGFR. Eps15 is required in clathrin mediated endocytosis including of synaptic vesicles. This protein has been shown to regulate the endosomal trafficking of EGFR, possibly through the recruitment of the ubiquitinated receptors to the rims of clathrin-coated pits. The EPS15 gene yields two isoforms that are believed to reside in distinct subcellular locations and thus implicated in different aspects of endosomal trafficking.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable