SAB4200203

Anti-RAVER1 antibody, Mouse monoclonal

clone RAV1, purified from hybridoma cell culture

• Synonym(s): Anti-Ribonucleoprotein PTB-binding 1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: RAV1, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~80 kDa
• species reactivity: human, monkey
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): suitable; western blot (chemiluminescent): 1.0-2.0 μg/mL using HeLa or HepG2 cells extract
• isotype: IgG1
• UniProt accession no.: Q8IY67
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... RAVER1(125950)

Description

General description

Monoclonal Anti-RAVER1 (mouse IgG1 isotype) is derived from the hybridoma RAV1 produced by the fusion of mouse myeloma cells (NS1) and splenocytes from mouse immunized with a peptide corresponding to a fragment of human RAVER1. Raver1, also known as ribonucleoprotein PTB-binding 1, is a widely expressed multidomain protein, identified in two-hybrid screens by its ability to interact and colocalize with the cytoskeletal proteins -actinin and vinculin. The protein is composed of three RNA recognition motifs (RRM) and of nuclear localization and nuclear export signals, allowing it to shuttle between the nucleus and the cytoplasm. Raver1 also colocalizes with PTB/hnRNPI.

Application

Monoclonal Anti-RAVER1 antibody produced in mouse has been used in immunoblotting.

Biochem/physiol Actions

RAVER1 (ribonucleoprotein, PTB binding 1) is involved in RNA splicing of microfilament proteins. In skeletal muscle, a translocation of Raver1 from the nucleus to the cytoplasm is correlated with the differentiation of specific microfilament attachment sites. Based on an analysis of Vinculin-Raver1 crystal structure it was suggested that vinculin recruits raver1 and its mRNAs cargo to focal adhesions, promoting localization of the synthesis of adhesion complexes by the translational machinery.

Physical form

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable