SAB4200139

Anti-VILIP-1 (C-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

• Synonym(s): Anti-HLP3, Anti-HPCAL3, Anti-HUVISL1, Anti-Hippocalcin-like protein3, Anti-VILIP, Anti-VSNL1, Anti-Visinin-like protein-1 (VLP-1)
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~22 kDa
• species reactivity: mouse, human, rat
• packaging: antibody small pack of 25 μL
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1.5 mg/mL
• technique(s): immunoprecipitation (IP): 5-10 μg using mouse brain extracts (S1 fraction); western blot: 1.5-3.0 μg/mL using rat brain extracts (S1 fraction) and HEK-293T cell lysate overexpressing human VILIP-1
• UniProt accession no.: P62760
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... VSNL1(7447) ; mouse ... Vsnl1(26950) ; rat ... Vsnl1(24877)

Description

General description

Visinin-like protein-1 (VILIP-1) belongs to a large family of neuronal Ca²⁺ sensor (NCS) proteins. The VILIP subfamily includes VILIP-1, VILIP-2, VILIP-3, hippocalcin and neurocalcin-δ. VILIP-1 is primarily expressed in the brain, particularly in the hippocampus. NCS proteins possess four EF-hand Ca²⁺-binding motifs, that facilitate their association with lipid bilayers.

Application

Anti-VILIP-1 (C-terminal) antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Visinin-like protein-1 (VILIP-1) associates with the plasma membrane and Golgi membranes and modulates the signaling of cAMP and cGMP in neuronal cells. It upregulates the expression of functional α4β2 neuronal nicotinic acetylcholine receptors in hippocampal neurons. VILIP-1 modulates the activity of guanylyl cyclase B through clathrin dependent receptor cycling, supporting a role for VILIP-1 in membrane trafficking in the central nervous system (CNS). VILIP-1 is implicated in the pathology of CNS disorders including Alzheimer′s disease, schizophrenia and as a biomarker in ischemic stroke. VILIP-1 expression is regulated during induction of hippocampal synaptic plasticity, underlying learning and memory processes. Neuronal Ca²⁺ sensor (NCS) proteins are involved in a variety of Ca²⁺-dependent signal transduction processes in neurons.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable