S0941

Anti-SMAC/Diablo antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• MDL number: MFCD03097270
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~24 kDa by SDS-PAGE
• species reactivity: human, mouse
• technique(s): microarray: suitable; western blot: 1:1,000 using whole cell extract of the human acute lymphoma Jurkat cell line
• UniProt accession no.: Q9NR28
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... DIABLO(56616) ; mouse ... Diablo(66593)

Description

General description

Anti-Smac/DIABLO is developed in rabbit using a synthetic peptide corresponding to the C-terminus of human Smac/DIABLO-L. Second mitochondria-derived activator of caspase/direct inhibitors of apoptosis protein (IAP) binding protein with low pI (Smac/DIABLO) is a mitochondria-derived pro-apoptotic protein that is released from the mitochondria in response to apoptotic stimuli. It exists as two isoforms, Smac/DIABLO-L and a short isoform Smac/DIABLO-S, differing in their N-terminal sequences. Smac/DIABLO-L also contains an N-terminal signal sequence. It is highly expressed in adult testis, ovary, liver, kidney, spleen, prostate and heart. This gene is located on human chromosome 12q24.31.

Immunogen

synthetic peptide corresponding to the C-terminus of human Smac/DIABLO-L (amino acids 219-239) conjugated to KLH.

Application

Anti-SMAC/Diablo antibody produced in rabbit may be used in immunoblotting and immunohistochemistry.

Biochem/physiol Actions

Second mitochondria-derived activator of caspase/direct IAP binding protein with low pI (Smac/DIABLO) is a proapoptotic protein, which promotes the caspase activity of the initiator caspase 9 and the effectors caspase 3 and caspase-7 by neutralizing the inhibitory activity of the inhibitors of apoptosis proteins (IAPs).It promotes apoptosis by removing XIAP from the apoptotic protease activating factor-1 (Apaf-1)/caspase-9 apoptosome. The N-terminal AVPIA sequence in mature Smac/DIABLO is absolutely required for interaction with the baculovirus IAP repeat 3 (BIR3) domain of XIAP. Mutations that disrupt Smac/DIABLO dimer formation abrogate the XIAP-neutralizing activity of this molecule, suggesting that Smac/DIABLO dimerization is essential for its proapoptotic activity. It plays a key role intrinsic and extrinsic in apoptotic pathways. Smac mRNA is overexpressed in de novo acute leukemia (AL) patients.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month.For prolonged storage, freeze in working aliquots at−20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also notrecommended. If slight turbidity occurs upon prolongedstorage, clarify the solution by centrifugation beforeuse. Working dilutions should be discarded if not usedwithin 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.