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RAB0404

Sigma-Aldrich

Human Placental Growth Factor  ELISA Kit

for serum, plasma, cell culture supernatant and urine

Synonym(s):

PIGF, Placenta growth factor, PLGF

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type urine
sample type serum
sample type plasma
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2 pg/mL
standard curve range: 1.372-1000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... PGF(5228)

General description

The Human PGF (placenta growth factor) ELISA (Enzyme- Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PGF in serum, plasma, cell culture supernatants and urine.
The PGF (placental growth factor) gene codes for a proangiogenic factor and is mapped to human chromosome 14q24.3. PGF belongs to the vascular endothelial growth factor (VEGF) family of growth factors. It is predominantly expressed in placenta.

Immunogen

Recombinant Human PLGF

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human Placental Growth Factor ELISA Kit has been used to determine PGF in the medium culture waste and cellular suspension.

Biochem/physiol Actions

Reduced levels of placental growth factor (PGF) indicates restricted growth in the fetus. Increased stimulation of PGF is observed in cancers and hind limb ischemia and in wound healing. Hypoxic conditions upregulate PGF expression. Increased expression of PGF gene is observed in cutaneous T-cell lymphoma and it supports in the cell proliferation process.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8B - Non-combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Prospect of umbilical cord mesenchymal stem cell culture waste in regenerative medicine.
Pawitan J A, et al.
Journal of Global Pharma Technology (2017)
Chromosome duplication (14q) and the genotype phenotype correlation.
Sadr-Nabavi A and Saeidi M
International journal of fertility & sterility, 8(1), 95-95 (2014)
Decreased PGF may contribute to trophoblast dysfunction in fetal growth restriction.
Wu W, et al.
Reproduction (Cambridge, England) (2017)
Placental growth factor and vascular endothelial growth factor together regulate tumour progression via increased vasculature in cutaneous T-cell lymphoma.
Miyagaki T, et al.
Acta Dermato-Venereologica, 97(5), 586-592 (2017)
Akiyo Sekimoto et al.
Biochemical and biophysical research communications, 521(3), 769-774 (2019-11-11)
- Tadalafil, a long-acting phosphodiesterase 5 (PDE5) inhibitor, alleviates preeclampsia (PE), and decreases the fetal and infant deaths associated with fetal growth restriction (FGR) in phase II clinical trial. Recently, we demonstrated that tadalafil alleviates FGR and hypertension in the

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