R8257
Mlu I from Micrococcus luteus (lysodeikticus)
buffered aqueous glycerol solution
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Specificity
Recognition sequence: 5′-A/CGCGT-3′
Ligation and recutting results: After 2-10-fold Mlu I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Partially inactivated at 65 °C for 10 minutes.
Ligation and recutting results: After 2-10-fold Mlu I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Partially inactivated at 65 °C for 10 minutes.
Other Notes
Supplied with 10x Restriction Endonuclease Buffer SH (B3657).
Caution
Comment: Mlu I will only partially cleave DNA isolated from E. coli strains that have the dam methylase (dam+ strains).
Physical form
Solution in 20 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 250 mM KCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.2% Triton X-100 (v/v) at 4°C
related product
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
New restriction endonucleases from Flavobacterium okeanokoites (FokI) and Micrococcus luteus (MluI).
H Sugisaki et al.
Gene, 16(1-3), 73-78 (1981-12-01)
Two new restriction endonucleases have been isolated from Flavobacterium okeanokoites IFO12536 and Micrococcus luteus IFO12992 and named FokI and MluI, respectively. Based on analysis of the sequences around the restriction sites, the recognition sequences and cleavage sites of these endonucleases
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
TALENs and ZFNs are associated with different mutation signatures.
Yongsub Kim et al.
Nature methods, 10(3), 185-185 (2013-02-12)
Cong Zhu et al.
Nucleic acids research, 41(4), 2455-2465 (2013-01-11)
Zinc-finger nucleases (ZFNs) have been used for genome engineering in a wide variety of organisms; however, it remains challenging to design effective ZFNs for many genomic sequences using publicly available zinc-finger modules. This limitation is in part because of potential
Annabel A Ferguson et al.
Methods in molecular biology (Clifton, N.J.), 940, 87-102 (2012-10-30)
The generation of transgenic animals is an essential part of research in Caenorhabditis elegans. One technique for the generation of these animals is biolistic bombardment involving the use of DNA-coated microparticles. To facilitate the identification of transgenic animals within a
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service