Skip to Content
Merck
All Photos(1)

Key Documents

R6501

Sigma-Aldrich

Ribonuclease H from Escherichia coli

recombinant, expressed in E. coli, buffered aqueous glycerol solution, 1,000-4,000 units/mL

Sign Into View Organizational & Contract Pricing


About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.

recombinant

expressed in E. coli

form

buffered aqueous glycerol solution

mol wt

17.6 kDa

packaging

vial of ~30 units

concentration

1,000-4,000 units/mL

shipped in

dry ice

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

Application

Ribonuclease H from Escherichia coli has been used in a study to assess metallobiochemistry of the magnesium ion. [1] Ribonuclease H has also been used in a study to investigate selective inhibitors of HIV-1 reverse transcriptase associated Rnase H activity. [2]

Unit Definition

One unit hydrolyzes 1.0 nanomole RNA in 3H-labeled poly(A) • poly(dT) to acid soluble material in 20 min at 37 °C.

Physical form

Solution in 50% glycerol containing 20 mM Tris-HCl, pH 7.5, 100 mM KCl, 10 mM MgCl2, 0.1 mM EDTA, 0.1 mM DTT and 0.05 mg BSA per ml

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Jessica H Brehm et al.
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 55(5), 737-745 (2012-05-24)
It is not known how often mutations in the connection and ribonuclease H domains of reverse transcriptase (RT) emerge with failure of first-line antiretroviral therapy (ART) in subtype C human immunodeficiency virus type 1 (HIV-1) infection and how these mutations
Virginie Suchaud et al.
Bioorganic & medicinal chemistry letters, 22(12), 3988-3992 (2012-05-23)
We report herein the synthesis of a series of 3-hydroxyquinolin-2(1H)-one derivatives. Esters and amide groups were introduced at position 4 of the basis scaffold and some modulations of the benzenic moiety were performed. Most compounds presented selective inhibitory properties in
H W Huang et al.
European journal of biochemistry, 219(1-2), 253-260 (1994-01-15)
Ribonuclease H (Escherichia coli) contains one strong magnesium-binding site, as determined by metal-titration experiments monitored by high field 1H-NMR and also by direct titration calorimetry. Kinetic and thermodynamic parameters were evaluated by 25Mg-NMR and were as follows: dissociation constant Kd
Mateusz Mendel et al.
Cell, 184(12), 3125-3142 (2021-05-01)
The N6-methyladenosine (m6A) RNA modification is used widely to alter the fate of mRNAs. Here we demonstrate that the C. elegans writer METT-10 (the ortholog of mouse METTL16) deposits an m6A mark on the 3' splice site (AG) of the S-adenosylmethionine

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service