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PP0530

Sigma-Aldrich

GlycoProfile™ 2-AA Labeling Kit

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About This Item

EC Number:
204-287-5
UNSPSC Code:
12352200
NACRES:
NA.32

General description

The GlycoProfile™ labeling kits are designed for efficient labeling of N-linked, O-linked, and glycosyl­phosphatidyl­inositol (GPI) anchored glycans with either 2-aminobenzamide (2-AB) or 2-aminobenzoic acid (anthranilic acid; 2-AA). These small fluorophores increase the spectral absorption of glycans, improving the detection of labeled glycans by HPAE (high-performance anion exchange chromatography), HPLC, and ESI-MS (electrospray mass spectroscopy) methods. Binding is robust and the resulting dye-glycan conjugates are stable, with no degradation during post-labeling analysis.

Application

GlycoProfile™ 2-AA Labeling Kit has been used in the labeling of fluorescently derivatized N-glycans from chinese hamster ovary CHO cells.
While 2-AB is slightly less sensitive than 2-AA, it is suitable for downstream glycan analysis by HPAE, HPLC and ESI-MS. Although suitable for the same applications, 2-AA is better suited for SDS-PAGE.

Legal Information

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

Product No.
Description
  • Anthranilic acid (2-AA) 2 x 6
  • Dimethyl sulfoxide (DMSO) 2 x 350
  • Acetic acid, glacial 2 x 200
  • Reductant (sodium cyanoborohydride) 2 x 6

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H318

Precautionary Statements

P280 - P305 + P351 + P338

Hazard Classifications

Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

302.0 °F - closed cup

Flash Point(C)

150 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Weiguo Fang et al.
Plant physiology, 154(3), 1549-1557 (2010-09-15)
The symbiotic associations between rhizospheric fungi and plants have enormous environmental impact. Fungi are crucial to plant health as antagonists of pathogens and herbivores and facilitate the uptake of soil nutrients. However, little is known about the plant products obtained
Modulation of IgG1 immunoeffector function by glycoengineering of the GDP-fucose biosynthesis pathway
Kelly RM, et al.
Biotechnology and Bioengineering, 115(3), 705-718 (2018)

Articles

Glycoprofile™ Labeling Kits

Glycoprofile Labeling Kits efficiently label glycans for glycan analysis, offering flexibility in choice of labeling.

Glycoprofile™ Labeling Kits

Glycoprofile Labeling Kits efficiently label glycans for glycan analysis, offering flexibility in choice of labeling.

Glycoprofile™ Labeling Kits

Glycoprofile Labeling Kits efficiently label glycans for glycan analysis, offering flexibility in choice of labeling.

Glycoprofile™ Labeling Kits

Glycoprofile Labeling Kits efficiently label glycans for glycan analysis, offering flexibility in choice of labeling.

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