This enzyme is active in the presence of 1% Triton X-100 and also 0.1% SDS.
P2289
Endoproteinase Lys-C from Lysobacter enzymogenes
lyophilized powder
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Size/SKU | Availability | Price |
|---|
About This Item
CAS Number:
UNSPSC Code:
12352204
EC Number:
276-716-4
MDL number:
Pricing and availability is not currently available.
form
lyophilized powder
Quality Segment
analyte chemical class(es)
amino acids
packaging
vial of ≥3.0 units
storage temp.
2-8°C
General description
Endoproteinase Lys-C is an enzyme that preferentially cleaves at the carboxyl side of lysine residues. [1]
Application
Endoproteinase Lys-C from Lysobacter enzymogenes is useful in the determination of primary structures of proteins. [1] It has been used in a study to investigate the evidence for trisulfide bonds in a recombinant variant of a human IgG2 monoclonal antibody. [2]
Endoproteinase Lys-C has been used for the digestion monoclonal antibodies for disulfide bond assignment. [3]
Biochem/physiol Actions
Endoproteinase Lys-C inactivates the enzyme inositol monophosphatase by cleaving it at a single site directly after Lys 36.
Other Notes
One unit will hydrolyze 1.0 μmole of N-p-tosyl-Gly-Pro-Lys p-nitroanilide per min at pH 7.7 at 25 °C.
signalword
Danger
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
wgk
WGK 1
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
-
Is the protease Endoproteinase Lys-C from Lysobacter enzymogenes, Product P2289, active in the presence of detergents?
1 answer-
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
What is the molecular weight of Endoproteinase Lys-C from Lysobacter enzymogenes, Product P2289?
1 answer-
The molecular weight is 30,000 Daltons (30 kDa).
Helpful?
-
-
What amount of Endoproteinase Lys-C from Lysobacter enzymogenes, Product P2289, is present in the 3 unit package size?
1 answer-
This product is prepared from material with a specific activity of 150 units per mg. Therefore there is approximately 20 micrograms per vial.
Helpful?
-