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MWGF200

Sigma-Aldrich

Gel Filtration Markers Kit for Protein Molecular Weights 12,000-200,000 Da

Synonym(s):

Gel Filtration Protein Ladder

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.25

form

solid

technique(s)

liquid chromatography (LC): suitable

storage temp.

−20°C

General description

Gel filtration markers kit contains five vials of proteins and 1 vial of blue dextran for calibration.

Application

Gel filtration markers are used in protein chromatography and gel filtration chromatography. Gel filtration markers have been used to study glucocorticoid-induced lymphocytolysis as a model system for apoptosis within the immune system. Gel filtration markers have also been used to isolate Polygonatum odoratum lectin, which showed remarkable anti-HSV-II activity towards Vero cells, cytotoxicity towards human melanoma A375 cells and induced apoptosis in a caspase-dependent manner.

Kit Components Also Available Separately

Product No.
Description
SDS

  • A8531Albumin, bovine serum, 66,000 Da MW 1 mL/vialSDS

  • A8656Alcohol Dehydrogenase, yeast, 150,000 Da MW 1 mL/vialSDS

  • A8781β-Amylase, sweet potato, 200,000 Da MW 1 mL/vialSDS

  • D4772 Blue dextran, 2,000,000 Da MW 1 mL/vialSDS

  • C7025Carbonic Anhydrase, bovine erythrocytes 29,000 Da MW 1 mL/vialSDS

  • C7150Cytochrome c, horse heart, 12,400 Da MW 1 mL/vialSDS

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Preparation of lectin-vicilin nanoparticle conjugates using the carbodiimide coupling technique.
Ezpeleta, I., et al.
International Journal of Pharmaceutics, 142(2), 227-233 (1996)
Exploring codon optimization and response surface methodology to express biologically active transmembrane RANKL in E. coli.
Maharjan S, Singh B, Bok JD, et al.
PLoS ONE, 9(5), e96259-e96259 (2014)
Two basic proteins isolated from <I>Clerodendrum inerme</I> Gaertn. are inducers of systemic antiviral resistance in susceptible plants.
Prasad, V., et al.
Plant Science, 110(1), 73-82 (1995)
Suguru Oguri et al.
Bioscience, biotechnology, and biochemistry, 72(10), 2640-2650 (2008-10-08)
A cDNA encoding tomato fruit lectin was cloned from an unripe cherry-tomato fruit cDNA library. The isolated lectin cDNA contained an open reading frame encoding 365 amino acids, including peptides that were sequenced. The deduced sequence consisted of three distinct
Fiona McArthur et al.
Journal of bacteriology, 187(15), 5292-5300 (2005-07-21)
The core oligosaccharide component of the lipopolysaccharide can be subdivided into inner and outer core regions. In Escherichia coli, the inner core consists of two 3-deoxy-d-manno-octulosonic acid and three glycero-manno-heptose residues. The HldE protein participates in the biosynthesis of ADP-glycero-manno-heptose

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