The L-Lactate standard provided in this kit is prepared as a concentrated solution at 20 mM solution. Following dilution to the recommended working concentration the solution may not be stored. It is best prepared fresh.
MAK329
L-Lactate Assay Kit
sufficient for 100 colorimetric tests
Synonym(s):
L-Lactic Acid Assay Kit, Lactate Quantification Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.
Recommended Products
usage
sufficient for 100 colorimetric tests
input
cell
plasma
serum
solution(s) (collagen)
detection method
colorimetric
relevant disease(s)
endocrinological disorders, diabetes; cancer
storage temp.
−20°C
General description
Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable.
Application
The L-Lactate Assay Kit may be used for:
- Cancer Research
- Diabetes Research
- Endocrinological Disease Research
Features and Benefits
Sensitive Detection Range: Detect L-Lactate levels accurately across a wide range, from 0.05 mM to 2 mM, using only 20 μL of sample in a 96-well plate setup, ensuring highly sensitive analysis with minimal sample requirement.
Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a 20 minute room temperature reaction, reducing complexity and saving valuable time and effort.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a 20 minute room temperature reaction, reducing complexity and saving valuable time and effort.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Suitability
Suitable for lactate determination in serum, plasma, and cell media samples.
Principle
The Lactate Assay Kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.
Other Notes
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Storage Class Code
10 - Combustible liquids
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For the lactate concentration standard curve, lactate standard solutions have to be prepared. Can these solutions be frozen and used multiple times? This would save a lot of time per Assay.
1 answer-
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