Acidification of urine samples is necessary for longer-term storage. Oxalate demonstrates greater solubility under acidic conditions. Additionally, ascorbic acid (Vitamin C) in urine samples rapidly degrades into oxalate in non-acidified samples, so acidifying urine should help to prevent this. Given that the Working Reagent used in this assay is acidic, acidifying fresh samples prior to the assay should not be necessary.
MAK315
Oxalate Assay Kit
Oxalate Assay Kit
sufficient for 100 colorimetric tests
Synonym(s):
Oxalic Acid Detection Kit
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.
Recommended Products
detection method
colorimetric
relevant disease(s)
genitourinary disorders
storage temp.
−20°C
General description
Oxalate or Oxalic Acid is a metabolic breakdown product of the Kreb′s Cycle in eukaryotes, and the glyoxylate cycle in other microorganisms. It can be found in the urine of humans and other mammals. Oxalate concentration can be used as a measure of kidney function where a high level of oxalate is an indicator for kidney stones which are primarily made of the insoluble salt calcium oxalate. Measuring oxalate is more accurate than measuring calcium as a marker for kidney stones because calcium is excreted at high concentrations even in normal urine.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for the quantitative determination of oxalate (oxalic acid) in urine, animal and plant tissue samples.
Principle
Simple, direct and high-throughput assays for measuring oxalate concentration find wide applications. This oxalate assay kit uses a single Working Reagent that combines the oxalate oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 595 nm is directly proportional to oxalate in the sample.
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Natalia Stepanova et al.
The Korean journal of internal medicine (2021-06-26)
It was hypothesized that oxalate might be strongly involved in atherogenesis and the inflammatory pathway that could result in an increased risk of cardiovascular disease (CVD) in end-stage renal disease (ESRD) patients. Therefore, this study aimed to address two primary
Natalia Stepanova et al.
In vivo (Athens, Greece), 36(2), 925-933 (2022-03-05)
Little evidence is available on oxalate balance in peritoneal dialysis (PD) patients. We performed a cross-sectional observational pilot study with 62 adult PD patients to document oxalate balance and explore its association with PD-related peritonitis. Plasma oxalate concentration, levels of
Natalia Stepanova et al.
Urolithiasis, 50(3), 249-258 (2022-02-08)
The present study aimed (i) to evaluate whether ceftriaxone treatment could affect not only intestinal oxalate-degrading bacteria number but also their total activity to degrade oxalate and influence oxalate homeostasis in rats, (ii) and to estimate the ability of commercially
Anna Kletzmayr et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 7(8), 1903337-1903337 (2020-04-25)
Calcium oxalate (CaOx) crystal-induced nephropathies comprise a range of kidney disorders, for which there are no efficient pharmacological treatments. Although CaOx crystallization inhibitors have been suggested as a therapeutic modality already decades ago, limited progress has been made in the
-
What is the purpose of acidifying the samples for longer-term storage?
1 answer-
Helpful?
-
-
What is the approximate expiration time for these kits?
1 answer-
Expiration dating is batch specific and should be determined from the lot specific Certificate of Analysis. Please review your batch specific Certificate of Analysis by searching your lot number in the DOCUMENTATION section of the Product Detail Page:
https://www.sigmaaldrich.com/product/sigma/MAK315#product-documentationHelpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
