Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

MAK242

Sigma-Aldrich

Thrombin Activity Fluorometric Assay Kit

sufficient for 100 fluorometric tests

Synonym(s):

Thrombin Enzyme Activity Kit

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

detection method

fluorometric

relevant disease(s)

hematological disorder

storage temp.

−20°C

General description

Thrombin enzyme (Factor IIa, EC 3.4.21.5), a Serine protease, is an important clotting factor in the coagulation cascade that involves the conversion of soluble fibrinogen to insoluble active fibrin strands. In this pathway, Prothrombin is proteolytically converted into an active Thrombin.[1][2]

Suitability

Suitable for determining the activity of pure Thrombin and for detecting the activity of Thrombin in plasma.

Principle

Thrombin activity fluorometric assay kit utilizes the ability of thrombin to proteolytically cleave a synthetic substrate and release a fluorophore, 7-amino-4-methylcoumarin (AMC), which can be easily quantified by fluorescence. This assay kit is simple, rapid and can detect thrombin activity as low as 1ng in samples.

Storage Class Code

10 - Combustible liquids

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
7B24K03730
7H26K03730
SLCC7286

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 2

1 of 2

Thrombin human BioUltra, recombinant, expressed in HEK 293 cells, aqueous solution, ≥95% (SDS-PAGE)

Sigma-Aldrich

T9326

Thrombin human

Thrombin from human plasma

Roche

10602400001

Thrombin

The conversion of prothrombin to thrombin I. Characterization of the reaction products formed during the activation of bovine prothrombin.
Owen W G, et al.
The Journal of Biological Chemistry, 249(2), 594-605 (1974)
Crystal structure of the thrombin-hirudin complex: a novel mode of serine protease inhibition.
Grutter M G, et al.
The Embo Journal, 9(8), 2361-2365 (1990)

Questions

  1. Hey just wondering how should we detect the AMC fluorophore released by the substrate? We have flow cytometer in the lab, but not sure how we can detect it.

    1 answer

    1. Please review the protocol at the link below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/176/154/mak242bul.pdf

      This kit is not intended for use in flow cytometry. A typical measurement is done on a plate reader like those presented in the link below:
      https://www.sigmaaldrich.com/substance/benchmarksmartreader96microplateabsorbancereaderwithincubation1234598765

      To discuss further, please navigate to the link https://www.sigmaaldrich.com/techservice, and click on "Product Technical Inquiries" under the Products Section with all the required information so that a member of the Technical Service team can reach out to assist further. Thank you.

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service