Skip to Content
Merck
All Photos(3)

Documents

MAK068

Sigma-Aldrich

MPO Colorimetric Activity Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

Myeloperoxidase Colorimetric Activity Assay Kit

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

cancer; cardiovascular diseases; hematological disorder

storage temp.

−20°C

Gene Information

human ... MPO(4353)
mouse ... MPO(17523)
rat ... MPO(303413)

General description

Myeloperoxidase (MPO) is a heme-containing enzyme that catalyzes the hydrogen peroxidase-mediated oxidation of halide ions to hypochlorous acid. MPO is a lysosomal protein, highly expressed in neutrophils, that plays a role in the antimicrobial actions that occur as a result of neutrophil stimulation. The actions of MPO may also contribute to the initiation and pathogenesis of cardiovascular disease.

Application

MPO Colorimetric Activity Assay Kit has been used to determine the activity of myeloperoxidase in samples.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the measurement of myeloperoxidase (MPO) in biological samples such as tissue, cells, serum and white blood cells

Principle

In this assay, MPO catalyzes the formation of hypochlorous acid, which reacts with taurine to form taurine chloroamine. Taurine chloroamine reacts with the chromophore TNB, resulting in the formation of the colorless product DTNB. One unit of MPO activity is defined as the amount of enzyme that hydrolyzes the substrate and generates taurine chloramine to consume 1.0 μmole of TNB per minute at 25 °C.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H315,H317,H319,H334

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Plasma nitration of high-density and low-density lipoproteins in chronic kidney disease patients receiving kidney transplants.
Bakillah A, et al.
Mediators of Inflammation, 2015 (2015)
Ruisong Pei et al.
Molecular nutrition & food research, 63(5), e1800985-e1800985 (2018-12-07)
Increased fruit consumption is associated with reduced risk of colitis. It has been investigated whether the anti-colitic effects of the polyphenol-rich aronia berry (Aronia mitschurinii 'Viking') are mediated through Th17 and Treg. Colitis is induced in recombinase activating gene-1 deficient
Yannan Liu et al.
American journal of physiology. Gastrointestinal and liver physiology, 307(5), G550-G563 (2014-07-19)
Understanding the regulation of death pathways, necrosis and apoptosis, in pancreatitis is important for developing therapies directed to the molecular pathogenesis of the disease. Protein kinase Cε (PKCε) has been previously shown to regulate inflammatory responses and zymogen activation in
MC-PPEA as a new and more potent inhibitor of CLP-induced sepsis and pulmonary inflammation than FK866
Huang P, et al.
Drug design, development and therapy, 11, 629-629 (2017)
Mary Caitlin P Sok et al.
Acta biomaterialia, 53, 109-122 (2017-02-19)
Many goals in tissue engineering rely on modulating cellular localization and polarization of cell signaling, including the inhibition of inflammatory infiltrate, facilitation of inflammatory cell egress, and clearance of apoptotic cells. Omega-3 polyunsaturated fatty acid-derived resolvins are gaining increasing recognition
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service